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  • Springer  (14)
  • Berlin : Max-Planck-Inst. für Infektionsbiologie  (1)
  • Berlin, Heidelberg :Springer Berlin / Heidelberg,  (1)
  • Göttingen :V&R Unipress,
Document type
Language
  • 1
    Keywords: Forschungsbericht
    Description / Table of Contents: Zweiphasensytem, attenuirte Salmonellen, DNA-Vektor, genetische Vakzinierung, T7-Polymerase, Gen E
    Type of Medium: Online Resource
    Pages: Online-Ressource (12 p., 142 Kb.)
    Edition: [Elektronische Ressource]
    Language: German
    Note: Contract BMBF 01 GE 9630 1. - Differences between the printed and electronic version of the document are possible. - German abstract under title: Improvement of the T7 expression system by the use of T7 lysozyme. - nIndex p. 7 - 11 , Also available as printed version , Systemvoraussetzungen: Acrobat Reader.
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  • 2
    Online Resource
    Online Resource
    Berlin, Heidelberg :Springer Berlin / Heidelberg,
    Keywords: Floodplains-Europe, Central. ; Riparian ecology-Europe, Central. ; Electronic books.
    Type of Medium: Online Resource
    Pages: 1 online resource (166 pages)
    Edition: 1st ed.
    ISBN: 9783662554555
    DDC: 570
    Language: German
    Note: Intro -- Vorwort -- Inhaltsverzeichnis -- 1 Grundwasserlebensräume in Auenwäldern -- 2 Quellen in Auenlandschaften -- 3 Geomorphologie von Flussläufen -- 4 Böden in Auenwäldern -- 5 Flussbegleitende Moore und Sümpfe -- 6 Hydromorphologie in Auenbiotopen -- 7 Paläoökologie der Auenbiotope -- 8 Pflanzengesellschaften in Auenbiotopen -- 9 Entomofauna in Auenbiotopen -- 10 Auentypische Mollusken und Krebstiere -- 11 Ichthyofauna der Auengewässer -- 12 Herpetofauna in Auen -- 13 Auentypische Avifauna -- 14 Säugetierfauna in Auenwäldern -- 15 Auenrevitalisierung -- Serviceteil -- Glossar auenökologischer Fachbegriffe -- Stichwortverzeichnis.
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  • 3
    Keywords: Electronic books.
    Type of Medium: Online Resource
    Pages: 1 online resource (309 pages)
    Edition: 1st ed.
    ISBN: 9783847016793
    Series Statement: Poetik, Exegese und Narrative / Poetics, Exegesis and Narrative
    Language: German
    Note: Intro -- Title Page -- Copyright -- Table of Contents -- Bettina Bannasch / George Y. Kohler: Vorwort der Herausgeber -- Body -- Hans-Joachim Hahn (Basel): Lessing, Dohm, Humboldt: Aspekte universalistischer Emanzipation -- I. Prolog -- II. Drei preußische Emanzipatoren und der universalistische Humanismus -- III. Lessing und das „Scheitern der Aufklärung" -- IV. Dohms Schrift und die ‚Dialektik der Aufklärungˋ -- V. Fazit -- Literaturverzeichnis -- Ze'ev Strauss (Hamburg): „Erst Judentum, dann Bildung". Samson Raphael Hirschs erziehungsprogrammatische Auseinandersetzung mit Naftali Herz Wessely -- 1. Einleitung -- 2. Die familiäre Prägung von Hirschs positivem Wesselybild -- 3. Hirschs Bezugnahmen auf Wessely in seinen Kommentaren -- 4. Umkehrung der religionspädagogischen Dialektik von Talmud Tora und Derekh Ereṣ -- 5. Fazit -- Literaturverzeichnis -- Asher Biemann (University of Virginia): Die Pflicht zur Selbstachtung: Emanzipation und jüdisches Selbstvertrauen bei Hermann Cohen -- 1. Dämmerung der Pflichten -- 2. Pflicht und Tugend -- 3. Staat und Recht -- 4. Treue und Vertrauen -- 5. Die Mündigkeit des Selbst und die Mündigkeit des Staates -- 6. Erhaltung und Emanzipation -- Literaturverzeichnis -- George Y. Kohler (Ramat Gan, Israel): Kantianische Irrtümer und freiheitlicher Sozialismus - Hermann Cohens Einfluss auf Kurt Eisner -- Kant als Urheber des Sozialismus -- ‚Regulative Idealisierungˋ -- Das Beispiel Karl Vorländer -- Kautsky und Tönnies gegen Cohen -- Eisner über Kant und Sozialismus -- Wer sind „jene Kantianer"? -- Literaturverzeichnis -- Bettina Bannasch (Augsburg): Wege ins Freie. Geschlechterspezifische Reflexionen des jüdischen Emanzipationsdiskurses in der Unterhaltungsliteratur um 1900 am Beispiel Auguste Hauschners -- 1. Emanzipation in der deutsch-jüdischen Unterhaltungsliteratur des 19. Jahrhunderts. , 2. Dekadente Verfalls- und emanzipatorische Fortschrittsnarrative im (jüdischen) Roman um 1900: Hauschners Familienromane im literaturgeschichtlichen Kontext -- 3. Die ‚natürlicheˋ Emanzipation der Kinder- von der Elterngeneration im Familien- und Generationenroman -- 4. Coming to an Understanding. Emanzipation von politischen und religiösen (Er)‍Lösungsszenarien -- 5. Hauschners Familienromane als kritischer Beitrag zur Auseinandersetzung um die sogenannte jüdische Emanzipation um 1900 -- Literaturverzeichnis -- Theresia Dingelmaier (Augsburg): Jüdische Renaissance‍(n) in deutschsprachiger jüdischer Kinderliteratur der Weimarer Republik - Zionistische Abenteuergeschichten, neue jüdische Kindermärchen und illustrierte Kinderbücher -- I. Einleitung -- II. Die Jüdische Renaissance -- III. Jüdische Renaissance‍(n) in deutschsprachigen jüdischen Texten für Kinder -- IV. … und noch ein Blick in die Gegenwart -- Literaturverzeichnis -- Abbildungsverzeichnis -- Anika Reichwald (Hohenems): Zwischen Welten - Ausgestelltes Judentum im deutschsprachigen Europa um 1900 -- Einleitung -- I. Ausgestelltes Judentum? - Judaica in (internationalen) Ausstellungen im späten 19. und frühen 20. Jahrhundert -- Die Sammlung Strauss und die Pariser Weltausstellung 1878 -- Judaica in weiteren internationalen Ausstellungen -- II. Zwischen Tradition und Moderne - Ausstellungen von, mit und über Judaica um 1900 -- Jüdische Kultur sammeln und ausstellen -- Die Inszenierung jüdischer Kultur -- III. Die ersten Jüdischen Museen - Schauplätze der Utopie von Zugehörigkeit‍(en)? -- Die Politik des Sammelns und Bewahrens - und ihre Umsetzung in den ersten Jüdischen Museen -- Zur Frage der Zugehörigkeit‍(en) Jüdischer Museen -- Forderungen zur Gründung eines Jüdischen National- oder Zentralmuseums -- Schlussbetrachtung -- Literaturverzeichnis -- Abbildungsverzeichnis. , Małgorzata Stolarska-Fronia (Potsdam): Der mystische Osten und der apokalyptische Westen. Verbindungen zwischen jüdischen Expressionisten aus Polen, der Ukraine und Deutschland anhand des Gästebuchs von Jakob Steinhardt -- Die Pathetiker - jüdische Avantgarde in Berlin -- Das Gästebuch von Minni und Jakob Steinhardt -- Zusammenfassung -- Literaturverzeichnis -- Abbildungsverzeichnis -- Julia Ingold (Bamberg): „Ich habe durch die Prinzenkrone nur dem Judentum einen Opal in die Schläfe gesetzt." Else Lasker-Schüler und die Popularität jüdischer Mystik im frühen 20. Jahrhundert -- 1. „Adelsbewußtsein" -- 2. Subversion -- 3. Gattungstraditionen -- Literaturverzeichnis -- Abbildungsverzeichnis -- Birgit R. Erdle (Berlin): Beobachtungsort postemanzipatorischer Lagen. Siegfried Kracauers Bestandsaufnahmen in der Zeit der Weimarer Republik -- 1. Situierte Sprachkritik -- 2. Universeller und historisierender Emanzipationsbegriff -- 3. Kracauers Deutung der Diaspora: Messianismus und jüdische Ethik -- 4. Rückschau auf die europäische Welt: Kracauer liest Die Flucht ohne Ende -- 5. Ein Denkbild: von Nationalismus, Assimilation, monolingualen Universen -- Literaturverzeichnis -- Dominique Hipp (Bonn): Entwürfe einer postmigrantischen Gesellschaft im journalistischen Werk von Joseph Roths Berliner Zeit -- 1. Eine kurze Geschichte des Feuilletons -- 2. Der Begriff des Postmigrantischen und das Feuilleton -- 3. Joseph Roth als Feuilletonist -- 3.1 Joseph Roths Erzählen über den Fakir als ein postmigrantisches -- 3.2 Postmigrantisches in den Berliner Feuilletons von Joseph Roth -- 4. Kulturkonservativ und trotzdem postmigrantisch? -- Literaturverzeichnis -- Liliane Weissberg (University of Pennsylvania): Zwischen Wien und Rom: Emanuel Löwy, Sigmund Freud und die Rolle der Erinnerung -- 1. Eine langjährige Freundschaft -- 2. Physiologie und antike Kunst. , 3. Nachträglichkeiten -- 4. Antiquitäten -- 5. Archäologische Schichten -- Literaturverzeichnis -- Thomas Meyer (Berlin/München): Hannah Arendts Umgänge mit dem Religionsbegriff -- 1. Erste Sichtung -- 2. Zweite Sichtung -- 3. Nachbemerkung -- Literaturverzeichnis.
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  • 4
    ISSN: 1432-1335
    Keywords: Estrogen receptor ; Estrogenic plantinum(II) complex ; Luciferase ; Transfection ; Drug stability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A rapid assay system for measuring the potential of estrogenic drugs is introduced. Luciferase induction could be measured in estrogen-receptor-positive human MCF-7 breast cancer cells, which had been transfected with a novel luciferase reporter plasmid ERE luc. The minimal requirement was 1 h exposure to the inducing drug and 3.5 h of incubation after removal of the drug. The assay system was used to measure the stability of the drug diaqua-[1,2-bis (2,6-dichloro-4-hydroxyphenyl) ethylenediamine] platinum(II) sulfate, containing an estrogenic ligand and reactive platinum. Luciferase activity was observed only when the drug was in the culture medium and cells for short times, whereas the estrogenic ligand alone remained active. It is assumed that binding of the platinum moiety to macromolecular constituents of the culture or cells renders the drug inaccessible for binding to the estrogen receptor.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Journal of computer aided molecular design 14 (2000), S. 403-409 
    ISSN: 1573-4951
    Keywords: cyclin-dependent kinase ; CDK1 ; inhibitor ; olomoucine ; structure-based design
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Cyclin-dependent kinase 1 (CDK1), an enzyme participating in the regulation of the cell cycle, constitutes a possible target in the search for new antitumor agents. Starting from the purine derivative olomoucine and following a structure-based approach, potent inhibitors of this enzyme were rapidly identified. The molecular modeling aspects of this work are described.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Molecular genetics and genomics 250 (1996), S. 277-285 
    ISSN: 1617-4623
    Keywords: Key words Gonococcus ; Folic acid ; Dihydrofolate synthetase ; Folylpolyglutamate synthetase ; One-carbon metabolism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The gene coding for folylpoly-(γ)-glutamate synthetase (FPGS)-dihydrofolate synthetase (DHFS) of Neisseria gonorrhoeae (Ngo) has been cloned by functional complementation of an Escherichia coli folC mutant (SF4). The sequence encodes a 224-residue protein of 46.4 kDa. It shows 46% identity to the E. coli FPGS-DHFS and 29% identity to the FPGS of Lactobacillus casei. Sequence comparisons between the three genes reveal regions of high homology, including ATP binding sites required for bifunctionality, all of which may be important for FPGS activity. In contrast to L. casei FPGS, the E. coli and Ngo enzymes share some additional regions which may be essential for DHFS activity. The products of Ngo folC and flanking genes were monitored by T7 promoter expression. Interestingly, deletion of the upstream folI gene, which encodes a 16.5 kDa protein, abolishes the capacity of folC to complement E. coli SF4 to the wild-type phenotype. The ability to complement can be restored by folI provided in trans. Unlike folC mutants, gonococcal folI mutants are viable and display no apparent phenotype. Thus, in contrast to E. coli, Ngo folC is expressed at a sufficiently high level from its own promoter, in the absence of FolI. This study provides the first insights into the genetic complexity of one-carbon metabolism in Ngo.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1617-4623
    Keywords: Plasmid vector ; Conjugation ; Generalized mutagenesis ; Homologous recombination ; Natural transformation competence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A versatile shuttle system has been developed for genetic complementation with cloned genes of transformable and non-transformableNeisseria mutants. By random insertion of a selectable marker into the conjugativeNeisseria plasmidptetM25.2, a site within this plasmid was identified that is compatible with plasmid replication and with conjugative transfer of plasmid. Regions flanking the permissive insertion site of ptetM25.2 were cloned inEscherichia coli and served as a basis for the construction of the Hermes vectors. Hermes vectors are composed of anE. coli replicon that does not support autonomous replication inNeisseria, e.g. ColE1, p15A, orori fd, fused with a shuttle consisting of a selectable marker and a multiple cloning site flanked by the integration region of ptetM25.2. Complementation of a non-transformableNeisseria strain involves a three-step process: (i) insertion of the desired gene into a Hermes vector; (ii) transformation of Hermes into aNeisseria strain containing ptetM25.2 to create a hybrid ptetM25.2 via gene replacement by the Hermes shuttle cassette; and (iii) conjugative transfer of the hybrid ptetM25.2 into the finalNeisseria recipient. Several applications for the genetic manipulation of pathogenicNeisseriae are described.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Molecular genetics and genomics 205 (1986), S. 494-500 
    ISSN: 1617-4623
    Keywords: Signal sequence ; Gene regulation ; Export ; Codon usage
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The nucleotide sequence of the ompV gene of Vibrio cholerae was determined. The product of the gene is a 28,000 dalton protein which, after the removal of a 19 amino acid signal sequence, produces a mature outer membrane protein of 26,000 daltons. The cleavage site was determined by amino-terminal amino acid sequencing of the purified mature protein. The DNA upstream of the gene shows the presence of a typical promoter region as judged from the Escherichia coli consensus information; however, the Shine-Dalgarno sequence is associated with a region capable of forming a secondary structure in the mRNA. The formation of this structure would inhibit binding of the mRNA to the ribosome and reduce translation. It is proposed that this structure is recognized by a positive activator in V. cholerae and because of its absence in E. coli ompV is poorly expressed. The distribution of rare codons within ompV suggests that they may serve to slow down the translation of particular domains such that the nascent polypeptide has an opportunity to take up its conformation without interference from the later formed regions. Such a mechanism could aid localization of the protein if export were by a cotranslational secretion system.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Molecular genetics and genomics 180 (1980), S. 489-494 
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Bacteriophage fd gene 2 protein was specifically labeled with radioactive amino acids and was isolated from membranous cell structures as an apparently homogenous protein. Amino acid sequence analysis revealed that the protein was initiated at two distinct AUG codons close to the ribosome binding site. The two resulting translation products were found to begin with a deformylated methionine residue. Initiation at the first signal was used for 90% of the chains and at the second signal for 10% of the sequenced molecules. The use of one or the other chain start may influence functions of gene 2 protein.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Molecular genetics and genomics 205 (1986), S. 501-506 
    ISSN: 1617-4623
    Keywords: Signal sequence ; Antigenic epitopes ; Outer membrane protein ; Immunogenicity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Fusion proteins comprising the amino-terminal 99 amino acids of the bacteriophage MS2 replicase and various portions of OmpV a major outer membrane protein of Vibrio cholerae were expressed in Escherichia coli K12. These fusions were expressed under the control of the PL promoter of bacteriophage λ, and expression was controlled using a cIts repressor. Fusions occurring within the secretory signal sequence of OmpV gave rise to the production of mature OmpV. The efficiency, however, decreased with progressive deletion of the signal sequence within the fusions. The reactivity of various OmpV fusions with antisera raised against purified OmpV and whole bacteria demonstrated the existence of two antigenic domains: one present in the denatured form and another in the membrane-associated form of OmpV. These domains correspond to markedly hydrophilic regions of the protein as would be predicted for surface-exposed epitopes.
    Type of Medium: Electronic Resource
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