In:
Current Stem Cell Research & Therapy, Bentham Science Publishers Ltd., Vol. 17, No. 5 ( 2022-07), p. 480-491
Abstract:
While bone marrow-derived mesenchymal stromal cells (BM-MSCs) have been
used for many years in bone tissue engineering applications, the procedure still has drawbacks such as painful collection methods and damage to the donor site. Dental pulp-derived stem cells (DPSCs) are
readily accessible, occur in high amounts, and show a high proliferation and differentiation capability. Therefore, DPSCs may be a promising alternative for BM-MSCs to repair bone defects. Objective: The aim of this study was to investigate the bone regenerative potential of DPSCs in comparison
to BM-MSCs in vitro and in vivo. Methods: In vitro investigations included analysis of cell doubling time as well as proliferation and osteogenic
differentiation. For the in vivo study, 36 male NMRI nude mice were randomized into 3 groups: 1) control (cell-free mineralized collagen matrix (MCM) scaffold), 2) MCM + DPSCs, and 3) MCM + BMMSCs.
Critical size 2 mm bone defects were created at the right femur of each mouse and stabilized by an external fixator. After 6 weeks, animals were euthanized, and microcomputed tomography scans (μCT)
and histological analyses were performed. Results: In vitro DPSCs showed a 2-fold lower population doubling time and a 9-fold higher increase in
proliferation when seeded onto MCM scaffolds as compared to BM-MSCs, but DPSCs showed a significantly lower osteogenic capability than BM-MSCs. In vivo, the healing of the critical bone defect in
NMRI nude mice was comparable among all groups. Conclusions: Pre-seeding of MCM scaffolds with DPSCs and BM-MSCs did not enhance bone defect
healing.
Type of Medium:
Online Resource
ISSN:
1574-888X
DOI:
10.2174/1574888X17666220215100732
Language:
English
Publisher:
Bentham Science Publishers Ltd.
Publication Date:
2022
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