In:
Current Genomics, Bentham Science Publishers Ltd., Vol. 21, No. 7 ( 2020-10-22), p. 504-511
Abstract:
In this study, whole genome re-sequencing of rust resistant soybean genotype
EC241780 was performed to understand the genomic landscape involved in the resistance mechanism. Methods: A total of 374 million raw reads were obtained with paired-end sequencing performed with
Illumina HiSeq 2500 instrument, out of which 287.3 million high quality reads were mapped to Williams 82 reference genome. Comparative sequence analysis of EC241780 with rust susceptible cultivars
Williams 82 and JS 335 was performed to identify sequence variation and to prioritise the candidate genes. Results: Comparative analysis indicates that genotype EC241780 has high sequence similarity with
rust resistant genotype PI 200492 and the resistance in EC241780 is conferred by the Rpp1 locus. Based on the sequence variations and functional annotations, three genes Glyma18G51715, Glyma18G51741
and Glyma18G51765 encoding for NBS-LRR family protein were identified as the most prominent candidate for Rpp1 locus. Conclusion: The study provides insights of genome-wide sequence variation more particularly at
Rpp1 loci which will help to develop rust resistant soybean cultivars through efficient exploration of the genomic resource.
Type of Medium:
Online Resource
ISSN:
1389-2029
DOI:
10.2174/1389202921999200601142258
Language:
English
Publisher:
Bentham Science Publishers Ltd.
Publication Date:
2020
detail.hit.zdb_id:
2044607-X
SSG:
12
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