In:
Current Drug Delivery, Bentham Science Publishers Ltd., Vol. 18, No. 7 ( 2021-08), p. 914-921
Abstract:
In the field of antibacterial, nanomaterials are favored by researchers because
of their unique advantages. Medicinal plants, especially traditional Chinese medicine, are considered to be an important source of new chemicals with potential therapeutic effects, as well as
an important source for the discovery of new antibiotics. MRSA is endangering people's lives as a kind of multidrug-resistant Staphylococcus aureus, which is resistant to tetracycline, amoxicillin,
norfloxacin and other first-line antibiotics. It is a hotspot to find good anti-drug-resistant bacteriae, nature-originated nanomaterials with good biocompatibility. Objective: We reported the formation of phytochemical nanoparticles (NPs) by the self-assembly
of berberine (BBR) and 3,4,5-methoxycinnamic acid (3,4,5-TCA) from Chinese herb medicine, which had good antibacterial activity against MRSA. Method and Results: We found that NPs had good antibacterial activity against MRSA; especially,
its antibacterial activity was better than first-line amoxicillin, norfloxacin and its self-assembling precursors on MRSA. When the concentration reached 0.1 μmol/mL, the inhibition rate of
NPs reached 94.62%, which was higher than that of BBR and the other two antibiotics (p 〈 0.001).
It was observed by Field-Emission Scanning Electron Microscopy (FESEM) that NPs could directly adhere to the bacterial surface, which might be an important aspect of the antibacterial activity
of NPs. Meanwhile, we further analyzed that the self-assembly was formed by hydrogen bonds and π-π stacking through Ultraviolet-Visible (UV-vis), Fourier Transform Infrared Spectroscopy (FTIR),
hydrogen Nuclear Magnetic Spectrum (1H NMR), and powder X-ray Diffraction (pXRD). NPs’ morphology was observed by FESEM and TEM. The particle size and surface charge were
characterized by Dynamic Light Scattering (DLS); and the surface charge was -31.6 mv, which proved that the synthesized NPs were stable. Conclusion: We successfully constructed a naturally self-assembled nanoparticle, originating from
traditional Chinese medicine, which had a good antibacterial activity for MRSA. It is a promising way to obtain natural nanoparticles from medicinal plants and apply them to antibacterial therapy.
Type of Medium:
Online Resource
ISSN:
1567-2018
DOI:
10.2174/1567201817666201124121918
Language:
English
Publisher:
Bentham Science Publishers Ltd.
Publication Date:
2021
SSG:
15,3
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