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  • 1
    Keywords: Electronic books.
    Type of Medium: Online Resource
    Pages: 1 online resource (88 pages)
    Edition: 1st ed.
    ISBN: 9786525252810
    Language: Portuguese
    Note: Intro -- Folha de Rosto -- Créditos -- APRESENTAÇÃO -- CAPÍTULO 1 Gênero Candida -- 1.1 CANDIDA AURIS -- CAPÍTULO 2 Antifúngicos -- 2.1 ANTIFÚNGICOS SINTÉTICOS -- Polienos -- Azoles (Triazoles) -- Equinocandinas (lipopeptídeos) -- Pirimidinas Fluoradas -- 2.2 ANTIFÚNGICOS NATURAIS -- CAPÍTULO 3 Espécies vegetais dos óleos essenciais -- 3.1 ÓLEOS ESSENCIAIS -- 3.2 ESPÉCIES VEGETAIS -- Cinamomum cassia (L.) J. Presl -- Eucalyptus citriodora Hooker -- Eucalyptus globulus Labill -- Eugenia caryophyllus Sprengel -- Melaleuca alternifolia Chell -- Myristica fragans Houtt -- Ocimum basilicum Linnaeus -- Origanum vulgare Linnaeus -- Rosmarinus officinalis Linnaeus -- Salvia sclarea Linnaeus -- Schinus terebinthifolius Raddi -- Thymus vulgaris Linnaeus -- Zingiber officinale Roscoe -- 3.3 METABÓLITOS SECUNDÁRIOS EM PLANTAS -- CAPÍTULO 4 Desenvolvimento da pesquisa -- 4.1 AMOSTRAS DE CANDIDA AURIS -- 4.2 ÓLEOS ESSENCIAIS E PRINCIPAIS COMPOSTOS QUÍMICOS -- 4.2.1 Compostos químicos dos óleos essenciais -- 4.3 PESQUISA DA ATIVIDADE DOS ÓLEOS ESSENCIAIS E PRINCIPAIS COMPOSTOS QUÍMICOS SOBRE C. auris -- 4.3.1 Preparações da suspensão de leveduras -- 4.3.2. Avaliação in vitro da atividade antifúngica dos óleos essenciais sobre isolados de C. auris -- 4.3.3 Avaliação in vitro da atividade antifúngica dos principais compostos químicos sobre isolados de C. auris -- 4.4 CONTROLES -- 4.5 DETERMINAÇÃO DA CONCENTRAÇÃO INIBITÓRIA MÍNIMA -- 4.6 AVALIAÇÃO DA ATIVIDADE FUNGISTÁTICA E FUNGICIDA DOS ÓLEOS ESSENCIAIS E DOS COMPOSTOS QUÍMICOS: -- 4.7 ENSAIO DA ATIVIDADE ANTIFÚNGICA DE ANFOTERICINA B, CETOCONAZOL, FLUCITOSINA, FLUCONAZOL, ITRACONAZOL E VORICONAZOL. -- 4.7.1 Método E-Test® - Kit Comercial (AB-Biodisk, Solna, Suécia) -- 4.7.1.1 Meio de cultivo -- 4.7.1.2 Fitas E-Test® -- 4.7.1.3 Preparo do Inóculo -- 4.7.1.4 Determinação da concentração inibitória mínima. , 4.7.1.5 Determinação e interpretação do teste -- 4.8 CONTROLES DE QUALIDADE E BIOSSEGURANÇA -- 4.9 DESCARTES DOS RESÍDUOS -- CAPÍTULO 5 Atividade dos óleos essenciais compostos químicos sobre Candida auris -- 5.1 Candida auris -- 5.2 ATIVIDADE DOS COMPOSTOS QUÍMICOS Α-HUMULENO A 96%, Β-CARIOFILENO A 80%, Γ-TERPINENO A > -- 95%, ALDEÍDO CINÂMICO A 99,97%, BORNEOL A 97,5%, CÂNFORA A 96%, CARVACROL A 98%, EUGENOL A 99%, ISOEUGENOL A > -- 98,9%, LINALOL A 97%, P-CIMENO A 99%, TERPINEOL A > -- 96%, TIMOL A 99,5% DE PUREZA, SOBRE AS CEPAS DE Candida auris -- 5.3 SENSIBILIDADE DA C. AURIS AOS ANTIFÚNGICOS SINTÉTICOS - ANFOTERICINA B, CETOCONAZOL, FLUCITOSINA, FLUCONAZOL, ITRACONAZOL E VORICONAZOL, UTILIZANDO AS FITAS E-TEST® -- CAPÍTULO 6 Panorama da atividade de óleos essenciais e compostos químicos sobre Candida auris -- 6.1 ATIVIDADES DE ÓLEOS ESSENCIAIS -- 6.1.1 Terpenos -- 6.1.2 Alcaloides -- 6.1.3 Fenóis -- 6.2 ATIVIDADE ENZIMÁTICA -- 6.3 ATIVIDADE DE ANTIFÚNGICOS SINTÉTICOS -- 6.4 OUTRAS CLASSES FARMACOLÓGICAS -- CAPÍTULO 7 Considerações finais.
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  • 2
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The importance of serum-free medium components on the growth of Chinese hamster ovary (CHO) cells and production of recombinant human interferon(IFN)-gamma was investigated. The complexity of the medium led to the adoption of a statistical optimization approach based on a Plackett-Burman design. From this analysis a set of nutritional components was identified as important for cell growth and recombinant protein production. Glycine was identified as an important determinant of specific growth rate, whereas for cell production bovine serum albumin (BSA), phenylalanine and tyrosine were also identified as important. BSA, sodium pyruvate, glutamate, methionine, proline, histidine, hydroxyproline, tyrosine and phenylalanine were shown to be important for IFN-gamma production. Other medium components, such as insulin, arginine, aspartate and serine produced an inhibitory effect on both cell growth and IFN-gamma production. The effect of the stimulatory nutrients as a whole group was tested by increasing their concentration in the medium. A significant improvement in specific cell growth rate, cell production and IFN-gamma production (up to 45%) was achieved on both shake-flask and fermentor cultures. An increase in the medium concentration of the negative variables had only a small inhibitory effect (approximately 10%) on the same parameters. Analysis of the effects of the group of stimulatory amino acids and BSA on CHO cell growth showed that the effect of the former was independent of BSA.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1573-0778
    Keywords: BSA ; CHO cells ; interferon-γ ; linoleic acid ; lipids ; Pluronic F68
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The role of bovine serum albumin in mammalian cell cultures and the possibility of its substitution by other components in a serum-free medium has been investigated. In this study, BSA was shown to be important for growth and product formation in CHO cells expressing recombinant human interferon-γ. There were indications that its stimulating growth effect was dependent on the source of BSA used and probably was related to the purification procedure used for the production of the desired albumin fraction. Cell growth did not occur in the absence of BSA but at low concentration (1 mg ml−1) it was stimulated by the addition of a combination of a commercial lipid mixture plus Pluronic F68. However, under the latter conditions IFN-γ production was adversely effected. The importance of individual lipid components was investigated using a statistical approach based on a Plackett-Burman design. Linoleic acid was identified as a positive variable for cell growth while cholesterol was identified as a negative variable for both cell growth and IFN-γ production. When a combination of linoleic acid plus Pluronic F68 was included in the formulation of low BSA medium, cell growth was similar to that at high BSA concentration (5 mg ml−1) but the IFN-γ concentration was significantly reduced (ca. 45%).
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1573-0778
    Keywords: Glycosylation ; recombinant ; interferon ; CHO cells ; lipids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The effects of lipids on the glycosylation of recombinant human interferon-γ expressed in a Chinese Hamster Ovary cell line were investigated in batch culture. Lipids form an essential part of the N-glycosylation pathway, and have been shown to improve cell viability. In control (serum-free) medium the proportion of fully-glycosylated interferon-γ deteriorated reproducibly with time in batch culture, but the lipoprotein supplement ExCyte was shown to minimise this trend. Partially substituting the bovine serum albumin content of the medium with a fatty-acid free preparation also improved interferon-γ glycosylation, possibly indicating that oxidised lipids carried on Cohn fraction V albumin may damage the glycosylation process.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Hydrobiologia 428 (2000), S. 171-177 
    ISSN: 1573-5117
    Keywords: fungal biomass ; leaf decomposition ; litterfall ; salt marsh ; Spartina maritima
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Spartina maritima (Curtis) Fernald is a dominant species in the Mondego salt marsh on the western coast of Portugal, and it plays a significant role in estuarine productivity. In this work, leaf litter production dynamics and fungal importance for leaf decomposition processes in Spartina maritima were studied. Leaf fall was highly seasonal, being significantly higher during dry months. It ranged from 42 g m-2 in June to less than 6 g m-2 during the winter. Fungal biomass, measured as ergosterol content, did not differ significantly between standing-decaying leaves and naturally detached leaves. Fungal biomass increased in wet months, with a maximum of 614 μg g-1 of ergosterol in January in standing-decaying leaves, and 1077 μg g-1 in December, in naturally detached leaves, decreasing greatly in summer. Seasonal pattern of fungal colonization was similar in leaves placed in litterbags on the marsh-sediment surface. However, ergosterol concentrations associated with standing-decaying and naturally detached leaves were always much higher than in litterbagged leaves, suggesting that fungal activity was more important before leaf fall. Dry mass of litterbagged leaves declined rapidly after 1 month (about 50%), mostly due to leaching of soluble organic compounds. After 13 months, Spartina leaves had lost 88% of their original dry weight. The decomposition rate constant (k) for Spartina maritima leaves was 0.151 month-1.
    Type of Medium: Electronic Resource
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