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  • Springer  (3)
  • Association for the Sciences of Limnology and Oceanography  (2)
  • Public Library of Science  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Book review (1983)
    Springer
    Microbial ecology 9 (1983), S. 77-77 
    Details
    ISSN: 1432-184X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02011582
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Observations on naturally and artificially diseased tropical corals: A scanning electron microscope study (1979)
    Springer
    Microbial ecology 5 (1979), S. 215-223 
    Details
    ISSN: 1432-184X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Scanning electron microscopic (SEM) observations of naturally and artificially diseased corals reveal that the disease is characterized by a filamentous matrix of cyanobacterial andBeggiatoa filaments. Spiral bacteria are commonly embedded in the matrix. The artificial disease is not manifested as the characteristic “black line disease” and does not contain filaments of cyanobacteria. This suggests that cyanobacteria are necessary for the black line phenomenon. The colorless, sulfide-oxidizing bacteriumBeggiatoa, however, is always associated with the disease.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02013528
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Book review (1980)
    Springer
    Microbial ecology 6 (1980), S. 185-187 
    Details
    ISSN: 1432-184X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02010558
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    Paper (German National Licenses)
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  • 4
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    Single-cell physiological structure and growth rates of heterotrophic bacteria in a temperate estuary (Waquoit Bay, Massachusetts) (2014)
    Association for the Sciences of Limnology and Oceanography
    Details
    Publication Date: 2022-05-26
    Description: Author Posting. © American Society of Limnology and Oceanography, 2011. This article is posted here by permission of American Society of Limnology and Oceanography for personal use, not for redistribution. The definitive version was published in Limnology and Oceanography 56 (2011): 37-48, doi:10.4319/lo.2011.56.1.0037.
    Description: Flow cytometric determinations of membrane integrity, nucleic acid content, and respiratory activity were combined with dilution cultures in Waquoit Bay Estuary (Massachusetts) to estimate specific growth rates of total, live, high (HNA), and low (LNA) nucleic acid content and actively respiring (CTC+) cells. Bacterial abundance ranged from 106 to 107 cells mL-1, with live cells generally contributing 〉 85% to total numbers, 42-82% HNA cells, and 3-36% CTC+ cells. Specific growth rates (µ) from all physiological groups were positively correlated, but they showed different temperature dependences, with activation energies ranging from 0.28 (live) to 0.97 eV (LNA). The µ values of live cells (0.14-2.40 d-1) were similar to those of total bacteria (0.06-1.53 d-1). LNA bacteria were not dormant but showed positive growth in most experiments, although HNA cells greatly outgrew LNA cells (µ ranges of 0.28-2.26 d-1 vs. 0-0.69 d-1), and CTC+ cells showed the highest values (0.12-2.65 d-1). Positive correlations of HNA bacteria µ with total and phytoplankton-derived dissolved organic carbon support the previously hypothesized strong bottom-up control of HNA cells. Bacterial production estimated from leucine incorporation and empirical conversion factors agreed well with estimates based on growth rates. HNA cells were always responsible for the largest share of bacterial production in the estuary. The contribution of CTC+ cells significantly increased with temperature in the 7-27°C range, reaching values of 40% at temperatures higher than 20°C.
    Description: This study was supported by the Spanish Ministry of Science and Innovation (MICINN) sabbatical stay program (to X.A.G.M.), National Science Foundation Office of Polar Programs grant 0823101 to H.W.D., and by the Marine Biological Laboratory.
    Repository Name: Woods Hole Open Access Server
    Type: Article
    Format: application/pdf
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  • 5
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    Ultrahigh bacterial production in a eutrophic subtropical Australian river : does viral lysis short-circuit the microbial loop? (2014)
    Association for the Sciences of Limnology and Oceanography
    Details
    Publication Date: 2022-05-26
    Description: Author Posting. © American Society of Limnology and Oceanography, 2011. This article is posted here by permission of American Society of Limnology and Oceanography for personal use, not for redistribution. The definitive version was published in Limnology and Oceanography 56 (2011): 1115-1129, doi:10.4319/lo.2011.56.3.1115.
    Description: We studied trophic dynamics in a warm eutrophic subtropical river (Bremer River, Australia) to determine potential sources of dissolved organic carbon (DOC) and the fate of heterotrophic bacterial production. Sustained high rates of bacterial production suggested that the exogenous DOC was accessible (labile). Bacterial specific growth rates (0.2 h−1 to 1.8 h−1) were some of the highest measured for natural aquatic ecosystems, which is consistent with high respiration rates. Bacteria consumed 10 times more organic carbon than that supplied by the daily algal production, a result that implies that terrestrial sources of organic carbon were driving the high rates of bacterial production. Viruses (1011 L−1) were 10 times more abundant than bacteria; the viral to bacterial ratio ranged from 3.5 to 12 in the wet summer and 11 to 35 in the dry spring weather typical of eutrophic environments. Through a combination of high bacterial respiration and phage lysis, a continuous supply of terrestrial DOC was lost from the aquatic ecosystem in a CO2-vented bacterial–viral loop. Bacterial processing of DOC in subtropical rivers may be contributing disproportionately large amounts of CO2 to the global carbon cycle compared to temperate freshwater ecosystems.
    Description: Thanks go to the Coastal Cooperative Research Centre and the Healthy Waterways Partnership for their funding.
    Repository Name: Woods Hole Open Access Server
    Type: Article
    Format: application/pdf
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  • 6
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    A method for studying protistan diversity using massively parallel sequencing of V9 hypervariable regions of small-subunit ribosomal RNA genes (2010)
    Public Library of Science
    Details
    Publication Date: 2022-05-26
    Description: © 2009 The Authors. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in PLoS ONE 4 (2009): e6372, doi:10.1371/journal.pone.0006372.
    Description: Massively parallel pyrosequencing of amplicons from the V6 hypervariable regions of small-subunit (SSU) ribosomal RNA (rRNA) genes is commonly used to assess diversity and richness in bacterial and archaeal populations. Recent advances in pyrosequencing technology provide read lengths of up to 240 nucleotides. Amplicon pyrosequencing can now be applied to longer variable regions of the SSU rRNA gene including the V9 region in eukaryotes. We present a protocol for the amplicon pyrosequencing of V9 regions for eukaryotic environmental samples for biodiversity inventories and species richness estimation. The International Census of Marine Microbes (ICoMM) and the Microbial Inventory Research Across Diverse Aquatic Long Term Ecological Research Sites (MIRADA-LTERs) projects are already employing this protocol for tag sequencing of eukaryotic samples in a wide diversity of both marine and freshwater environments. Massively parallel pyrosequencing of eukaryotic V9 hypervariable regions of SSU rRNA genes provides a means of estimating species richness from deeply-sampled populations and for discovering novel species from the environment.
    Description: This work was supported by grants from the W.M. Keck Foundation and the Woods Hole Center for Oceans and Human Health from the National Institutes of Health and National Science Foundation (NIH/NIEHS 1 P50 ES012742-01 and NSF/OCE 0430724-J) (LAZ and SH).
    Repository Name: Woods Hole Open Access Server
    Type: Article
    Format: application/pdf
    Format: image/tiff
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