In:
COLLOQUIUM AGRARIAE, Associacao Prudentina de Educacao e Cultura (APEC), Vol. 13, No. Especial 2 ( 2017-6-1), p. 124-128
Abstract:
The objective of the techniques of conservation of anatomical pieces is to preserve the
morphology, consistency, color and flexibility of the same. Among the techniques currently used are formaldehyde, glycerin and fill/corrosion, which were compared in the present study. Four
pieces were used in the evaluation of each technique. For formalinization, the lungs were washed, dissected and fixed in 10% formalin. For the glycerination technique, the organs were fixed in 10%
formaldehyde, washed for 48 hours, and then dried in the shade. Sequentially, the organs were submerged in hydrogen peroxide 10 and again dried in the shade. Then, they remained
submerged in absolute alcohol for two months, were removed, dried naturally, and submerged in glycerin for 30 days. For the filling/corrosion technique the lungs were washed, then submerged in
125 Colloquium Agrariae, vol. 13, n. Especial 2, Jan–Jun, 2017, p. 124-128. ISSN: 1809-8215. DOI: 10.5747/ca.2017.v13.nesp2.000217
alcohol for 24 hours. The trachea was cannulated for the fixation step, through the canula a continuous air stream containing alcohol and formalin mixture was introduced into the bronchial
tree. Subsequently, the acrylic was inserted, the pieces were placed in the water for 24 hours, and soon the corrosion was carried out with hydrochloric acid. The glycerolation technique proved to
be more advantageous when compared to formaldehyde, since it presents a low toxicity and irritation index, which facilitated the management and study, besides requiring a low degree of
maintenance. However, for the visualization of the bronchial tree, the filling/corrosion technique proved to be superior to the others, but this technique demands greater care during its handling
and storage.
Type of Medium:
Online Resource
ISSN:
1809-8215
DOI:
10.5747/ca.2017.v13.nesp2
DOI:
10.5747/ca.2017.v13.nesp2.000217
Language:
Unknown
Publisher:
Associacao Prudentina de Educacao e Cultura (APEC)
Publication Date:
2017
detail.hit.zdb_id:
2628994-5
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