In:
Animal Bioscience, Asian Australasian Association of Animal Production Societies, Vol. 36, No. 4 ( 2023-04-01), p. 555-569
Abstract:
Objective: The objective of this study was to investigate the effects of N6-Methyladenosine modification-circRNA-zinc finger protein 638 (m 〈 sup 〉 6 〈 /sup 〉 A-circRNA-ZNF638) on the induced activation of secondary hair follicle (SHF) stem cells with its potential mechanisms in cashmere goats.Methods: The m 〈 sup 〉 6 〈 /sup 〉 A modification of ZNF638 was analyzed using methylation immunoprecipitation with real-time quantitative polymerase chain reaction technique in SHF stem cells. The effects of circRNA-ZNF638 on the induced activation of SHF stem cells in m 〈 sup 〉 6 〈 /sup 〉 A dependence were evaluated through the overexpression of circRNA-ZNF638/its m6Adeficient mutants in circRNA-ZNF638 knockdown SHF stem cells. The competitive binding of miR-361-5p to circRNA-ZNF638/Wnt5a 3′- untranslated region was analyzed through Dual-luciferase reporter assay.Results: The m 〈 sup 〉 6 〈 /sup 〉 A-circRNA-ZNF638 had significantly higher transcription at anagen SHF bulge of cashmere goats compared with that at telogen, as well as it positively regulated the induced activation of SHF-stem cells in cashmere goats. Mechanismly, m 〈 sup 〉 6 〈 /sup 〉 A-circRNA-ZNF638 sponged miR-361-5p to heighten the transcriptional expression of 〈 i 〉 Wnt5a 〈 /i 〉 gene in SHFstem cells. We further demonstrated that the internal m 〈 sup 〉 6 〈 /sup 〉 A modification within circRNAZNF638 is required for mediating the miR-361-5p/Wnt5a pathway to regulate the induced activation of SHF stem cells through an introducing of m 〈 sup 〉 6 〈 /sup 〉 A-deficient mutant of circRNAZNF638.Conclusion: The circRNA-ZNF638 contributes the proper induced activation of SHF-stem cells in cashmere goats in m 〈 sup 〉 6 〈 /sup 〉 A-dependent manner through miR-361-5p/Wnt5a axis.
Type of Medium:
Online Resource
ISSN:
2765-0189
,
2765-0235
Language:
English
Publisher:
Asian Australasian Association of Animal Production Societies
Publication Date:
2023
detail.hit.zdb_id:
3055169-9
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