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  • American Society for Microbiology  (3)
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  • American Society for Microbiology  (3)
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  • 1
    Online Resource
    Online Resource
    An Adenovirus Type 5 Mutant with the Preterminal Protein Gene Deleted Efficiently Provides Helper Functions for the Production of Recombinant Adeno-Associated Virus
    American Society for Microbiology ; 1998
    In:  Journal of Virology Vol. 72, No. 10 ( 1998-10), p. 8371-8373
    Details
    In: Journal of Virology, American Society for Microbiology, Vol. 72, No. 10 ( 1998-10), p. 8371-8373
    Abstract: Production of recombinant adeno-associated virus (rAAV) requires helper functions that have routinely been provided by infection of the producer cells with adenovirus. Complete removal and/or inactivation of progeny adenovirus, present in such rAAV preparations, presents significant difficulty. Here, we report that an adenovirus type 5 (Ad5) mutant with the preterminal protein (pTP) gene deleted can provide helper function for the growth of rAAV. At high multiplicity, Ad5 dl 308 ΔpTP was as efficient as the phenotypically wild-type Ad5 dl 309 in permitting growth of rAAV. Use of Ad5 dl 308 ΔpTP , which is incapable of replication in the absence of complementation for pTP, as a helper avoids the need to remove contaminating adenovirus infectious activity by heat inactivation or by purification. Comparison of the transducing ability of rAAV generated with either Ad5 dl 308 ΔpTP or Ad5 dl 309 as a helper demonstrated that the heat inactivation protocol generally used does not remove all of the helper Ad5 dl 309 function.
    Type of Medium: Online Resource
    ISSN: 0022-538X , 1098-5514
    URL: Article
    DOI: 10.1128/JVI.72.10.8371-8373.1998
    Language: English
    Publisher: American Society for Microbiology
    Publication Date: 1998
    detail.hit.zdb_id: 1495529-5
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    Online Resource
  • 2
    Online Resource
    Online Resource
    Strand-Specific Transcription of Polyoma Virus DNA Early in Productive Infection and in Transformed Cells
    American Society for Microbiology ; 1976
    In:  Journal of Virology Vol. 17, No. 1 ( 1976-01), p. 20-26
    Details
    In: Journal of Virology, American Society for Microbiology, Vol. 17, No. 1 ( 1976-01), p. 20-26
    Abstract: The DNA strand origin of nuclear and cytoplasmic polyoma-specific RNA in productively infected mouse cells and in a line of polyoma-transformed hamster cells was determined by hybridization of unlabeled RNA with radioactively labeled separated strands of polyoma DNA. Early in the productive cycle (10 h postinfection) nuclear viral RNA is complementary to only about 40% of the E strand of viral DNA. No RNA complementary to the L strand was detected even when the RNA was first self-annealed to enrich for possible minor species. Early cytoplasmic RNA is complementary to the same 40% of the E strand. Thus, only that part of the polyoma genome which codes for early viral messenger RNA appears to be transcribed. Late in infection, nuclear viral RNA is complementary to most or all of the L strand and to at least 60% of the E strand. Late cytoplasmic viral RNA hybridizes to 40 to 45% of the E strand and 50 to 55% of the L strand. The transformed cell nuclear viral RNA is complementary to 60% of the E strand, whereas cytoplasmic RNA is complementary to 40% of the E strand and comprises the same polyoma-specific sequences as are found in RNA early in productive infection. No L strand transcripts could be detected. Thus, in the transformed cells and late in productive infection, viral RNA sequences in the cytoplasm are a specific subset of those in the nucleus.
    Type of Medium: Online Resource
    ISSN: 0022-538X , 1098-5514
    URL: Article
    DOI: 10.1128/jvi.17.1.20-26.1976
    Language: English
    Publisher: American Society for Microbiology
    Publication Date: 1976
    detail.hit.zdb_id: 1495529-5
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  • 3
    Online Resource
    Online Resource
    Enhancement of Antimicrobial Activity against Pseudomonas aeruginosa by Coadministration of G10KHc and Tobramycin
    American Society for Microbiology ; 2006
    In:  Antimicrobial Agents and Chemotherapy Vol. 50, No. 11 ( 2006-11), p. 3833-3838
    Details
    In: Antimicrobial Agents and Chemotherapy, American Society for Microbiology, Vol. 50, No. 11 ( 2006-11), p. 3833-3838
    Abstract: Pseudomonas aeruginosa is a common opportunistic human pathogen that is associated with life-threatening acute infections and chronic airway colonization during cystic fibrosis. Previously, we converted the wide-spectrum antimicrobial peptide novispirin G10 into a s electively- t argeted a nti m icrobial p eptide (STAMP), G10KHc. Compared to novispirin G10, the STAMP had an enhanced ability to kill Pseudomonas mendocina . In this study, we explored the activity of G10KHc against P. aeruginosa . G10KHc was found to be highly active (as active as tobramycin) against P. aeruginosa clinical isolates. Most interestingly, we observed a synergistic-like enhancement in killing activity when biofilms and planktonic cultures of P. aeruginosa were cotreated with G10KHc and tobramycin. The data indicate that the mechanism of enhanced activity may involve increased tobramycin uptake due to G10KHc-mediated cell membrane disruption. These results suggest that G10KHc may be useful against P. aeruginosa during acute and chronic infection states, especially when it is coadministered with tobramycin.
    Type of Medium: Online Resource
    ISSN: 0066-4804 , 1098-6596
    URL: Article
    DOI: 10.1128/AAC.00509-06
    RVK:
    XA 24552
    Language: English
    Publisher: American Society for Microbiology
    Publication Date: 2006
    detail.hit.zdb_id: 1496156-8
    SSG: 12
    SSG: 15,3
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