In:
Molecular Biology of the Cell, American Society for Cell Biology (ASCB), Vol. 10, No. 9 ( 1999-09), p. 2945-2953
Kurzfassung:
Dendritic mRNA transport and local translation at individual potentiated synapses may represent an elegant way to form synaptic memory. Recently, we characterized Staufen, a double-stranded RNA-binding protein, in rat hippocampal neurons and showed its presence in large RNA-containing granules, which colocalize with microtubules in dendrites. In this paper, we transiently transfect hippocampal neurons with human Staufen-green fluorescent protein (GFP) and find fluorescent granules in the somatodendritic domain of these cells. Human Stau-GFP granules show the same cellular distribution and size and also contain RNA, as already shown for the endogenous Stau particles. In time-lapse videomicroscopy, we show the bidirectional movement of these Staufen-GFP–labeled granules from the cell body into dendrites and vice versa. The average speed of these particles was 6.4 μm/min with a maximum velocity of 24.3 μm/min. Moreover, we demonstrate that the observed assembly into granules and their subsequent dendritic movement is microtubule dependent. Taken together, we have characterized a novel, nonvesicular, microtubule-dependent transport pathway involving RNA-containing granules with Staufen as a core component. This is the first demonstration in living neurons of movement of an essential protein constituent of the mRNA transport machinery.
Materialart:
Online-Ressource
ISSN:
1059-1524
,
1939-4586
DOI:
10.1091/mbc.10.9.2945
Sprache:
Englisch
Verlag:
American Society for Cell Biology (ASCB)
Publikationsdatum:
1999
ZDB Id:
1474922-1
SSG:
12
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