In:
Materials Express, American Scientific Publishers, Vol. 10, No. 9 ( 2020-09-30), p. 1554-1559
Abstract:
To investigate the neuroprotective effects of pretreatment with Fe 3 O 4 methylprednisolone plus calpain inhibitor against spinal cord ischemia-reperfusion injury (SCI) in rats. A total of 18 male Sprague Dawley rats were randomly divided into sham operation, model, and pretreatment + SCI groups (receiving 25 mg/kg Fe 3 O 4 methylprednisolone plus calpain inhibitor) ( n = 6 each). The SCI model was established by clamping the left renal inferior abdominal aorta. Hindlimb motor function was evaluated 6, 12, 24, and 48 hours after
reperfusion. At 48 hours after reperfusion, spinal cord tissues were collected for histopathological examination, measurement of oxidation product accumulation (malondialdehyde, MDA), and antioxidant enzyme activity assays [superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-PX)]. Motor function scores were compared using non-parametric rank sum (Kruskal-Wallis) test, and mean tissue MDA content and antioxidant enzyme activities were compared using one-way analysis of variance. Compared with the sham operation group, the model group showed significantly increased MDA content (2.95 vs. 5.90 g mg; P = 0.023); significantly increased GSH-Px activity (0.31 vs. 0.78 U/mg; P = 0.001); significantly decreased SOD (16.03 vs. 8.37 U/mg; P = 0.001) and CAT (1.24 vs. 0.54 U/mg; P = 0.002) activities; and significantly decreased hindlimb
motor function score at 6 hours (21 to 15.5; P = 0.001). Compared with the model group, the pretreatment + SCI group showed significantly decreased MDA content (5.90 vs. 3.27 mg; P = 0.0355); significantly decreased GSH-Px activity (0.78 vs. 0.36 U/mg; P = 0.001); and
significantly increased SOD (8.37 vs. 14.79 U/mg; P = 0.001) and CAT (0.54 vs. 0.89 U/mg; P = 0.043) activities. Histopathological changes were significantly less severe in the pretreatment + SCI group than in the model and sham operation groups ( P 〈 0.05). Pretreatment
with Fe 3 O 4 methylprednisolone plus calpain inhibitor can prevent SCI by improving the balance between oxidation and antioxidant systems.
Type of Medium:
Online Resource
ISSN:
2158-5849
DOI:
10.1166/mex.2020.1781
Language:
English
Publisher:
American Scientific Publishers
Publication Date:
2020
detail.hit.zdb_id:
2905325-0
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