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  • American Physical Society (APS)  (1)
  • GFZ Data Services  (1)
  • The American Society for Biochemistry and Molecular Biology (ASBMB)  (1)
  • 1
    Publication Date: 2012-09-01
    Description: The P5-ATPases are important components of eukaryotic cells. They have been shown to influence protein biogenesis, folding, and transport. The knowledge of their biochemical properties is, however, limited, and the transported ions are still unknown. We expressed in Saccharomyces cerevisiae the yeast Spf1 P5A-ATPase containing the GFP fused at the N-terminal end. The GFP-Spf1 protein was localized in the yeast endoplasmic reticulum. Purified preparations of GFP-Spf1 hydrolyzed ATP at a rate of ∼0.3–1 μmol of Pi/mg/min and formed a phosphoenzyme in a simple reaction medium containing no added metal ions except Mg2+. No significant differences were found between the ATPase activity of GFP-Spf1 and recombinant Spf1. Omission of protease inhibitors from the purification buffers resulted in a high level of endogenous proteolysis at the C-terminal portion of the GFP-Spf1 molecule that abolished phosphoenzyme formation. The Mg2+ dependence of the GFP-Spf1 ATPase was similar to that of other P-ATPases where Mg2+ acts as a cofactor. The addition of Mn2+ to the reaction medium decreased the ATPase activity. The enzyme manifested optimal activity at a near neutral pH. When chased by the addition of cold ATP, 90% of the phosphoenzyme remained stable after 5 s. In contrast, the phosphoenzyme rapidly decayed to less than 20% when chased for 3 s by the addition of ADP. The greater effect of ADP accelerating the disappearance of EP suggests that GFP-Spf1 accumulated the E1∼P phosphoenzyme. This behavior may reflect a limiting countertransported substrate needed to promote turnover or a missing regulatory factor.
    Print ISSN: 0021-9258
    Electronic ISSN: 1083-351X
    Topics: Biology , Chemistry and Pharmacology
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  • 2
    Publication Date: 2011-12-28
    Description: Author(s): O. A. Pinto, F. Nieto, and A. J. Ramirez-Pastor [Phys. Rev. E 84, 061142] Published Tue Dec 27, 2011
    Keywords: Statistical physics
    Print ISSN: 1539-3755
    Electronic ISSN: 1550-2376
    Topics: Physics
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  • 3
    Publication Date: 2020-05-19
    Description: Analyzing the chemical composition of rocks and minerals is an important tool for exploring and understanding mineral resources. Typically, hydrothermal ore deposits are characterized by primary alteration halos. At the world-class Panasqueira W-Sn-Cu deposit, the hydrothermal alteration of the wall rocks produced concentric zones with progressively greater distance from the veins, consisting of a proximal tourmaline-quartz-muscovite zone and a distal muscovite-quartz zone. Tourmaline and mica are ubiquitous minerals at Panasqueira W-Sn-Cu and coexist in many other hydrothermal ore deposits worldwide. Both minerals are well-known to host variable amounts of trace elements and to have potential as pathfinder minerals as well as fluid monitors. We analyzed major, minor and trace element contents of altered and unaltered metasediments from the Panasqueira by XRF and ICP-MS and tourmaline and white mica major, minor and trace element compositions by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) in previously well-characterized samples from different locations/setting in the mine (greisen, vein-selvages, wall-rock alteration zones, fault zone, and late vugs). Detailed information about the samples used, the location, and general geological background of the samples, and the analytical method is provided in the data description "2020-002_Codeco-et-al_data-description.pdf ". Detailed information about the the samples used, the location and general geological background of the samples and the analytical methods are provided in the data description file (2020-002_Codeco-et-al_data-description.pdf).
    Language: English
    Type: info:eu-repo/semantics/workingPaper
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