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  • 1
    Publication Date: 2022-01-31
    Description: Considerable advances in the global ocean observing system over the last two decades offers an opportunity to provide more quantitative information on changes in heat and freshwater storage. Variations in these storage terms can arise through internal variability and also the response of the ocean to anthropogenic climate change. Disentangling these competing influences on the regional patterns of change and elucidating their governing processes remains an outstanding scientific challenge. This challenge is compounded by instrumental and sampling uncertainties. The combined use of ocean observations and model simulations is the most viable method to assess the forced signal from noise and ascertain the primary drivers of variability and change. Moreover, this approach offers the potential for improved seasonal-to-decadal predictions and the possibility to develop powerful multi-variate constraints on climate model future projections. Regional heat storage changes dominate the steric contribution to sea level rise over most of the ocean and are vital to understanding both global and regional heat budgets. Variations in regional freshwater storage are particularly relevant to our understanding of changes in the hydrological cycle and can potentially be used to verify local ocean mass addition from terrestrial and cryospheric systems associated with contemporary sea level rise. This White Paper will examine the ability of the current ocean observing system to quantify changes in regional heat and freshwater storage. In particular we will seek to answer the question: What time and space scales are currently resolved in different regions of the global oceans? In light of some of the key scientific questions, we will discuss the requirements for measurement accuracy, sampling, and coverage as well as the synergies that can be leveraged by more comprehensively analysing the multi-variable arrays provided by the integrated observing system.
    Type: Article , PeerReviewed
    Format: text
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  • 2
    Publication Date: 2013-07-31
    Description: Nature Methods 10, 741 (2013). doi:10.1038/nmeth.2532 Authors: Ari E Friedland, Yonatan B Tzur, Kevin M Esvelt, Monica P Colaiácovo, George M Church & John A Calarco We report the use of clustered, regularly interspaced, short palindromic repeats (CRISPR)-associated endonuclease Cas9 to target genomic sequences in the Caenorhabditis elegans germ line using single-guide RNAs that are expressed from a U6 small nuclear RNA promoter. Our results demonstrate that targeted, heritable genetic alterations can be achieved in C. elegans, providing a convenient and effective approach for generating loss-of-function mutants.
    Print ISSN: 1548-7091
    Electronic ISSN: 1548-7105
    Topics: Biology , Medicine
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  • 3
    Publication Date: 2014-05-21
    Description: Ecogenomic sensor reveals controls on N2-fixing microorganisms in the North Pacific Ocean The ISME Journal 8, 1175 (June 2014). doi:10.1038/ismej.2013.244 Authors: Julie C Robidart, Matthew J Church, John P Ryan, François Ascani, Samuel T Wilson, Deniz Bombar, Roman Marin, Kelvin J Richards, David M Karl, Christopher A Scholin & Jonathan P Zehr
    Keywords: autonomous sensingbiosensorsdiazotrophsmicrobial oceanographynitrogen fixationtime-series
    Print ISSN: 1751-7362
    Electronic ISSN: 1751-7370
    Topics: Biology
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  • 4
    Publication Date: 2022-09-14
    Description: Author Posting. © American Meteorological Society, 2022. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Journal of Climate 35(2), (2022): 851–875, https://doi.org/10.1175/JCLI-D-20-0603.1.
    Description: The Earth system is accumulating energy due to human-induced activities. More than 90% of this energy has been stored in the ocean as heat since 1970, with ∼60% of that in the upper 700 m. Differences in upper-ocean heat content anomaly (OHCA) estimates, however, exist. Here, we use a dataset protocol for 1970–2008—with six instrumental bias adjustments applied to expendable bathythermograph (XBT) data, and mapped by six research groups—to evaluate the spatiotemporal spread in upper OHCA estimates arising from two choices: 1) those arising from instrumental bias adjustments and 2) those arising from mathematical (i.e., mapping) techniques to interpolate and extrapolate data in space and time. We also examined the effect of a common ocean mask, which reveals that exclusion of shallow seas can reduce global OHCA estimates up to 13%. Spread due to mapping method is largest in the Indian Ocean and in the eddy-rich and frontal regions of all basins. Spread due to XBT bias adjustment is largest in the Pacific Ocean within 30°N–30°S. In both mapping and XBT cases, spread is higher for 1990–2004. Statistically different trends among mapping methods are found not only in the poorly observed Southern Ocean but also in the well-observed northwest Atlantic. Our results cannot determine the best mapping or bias adjustment schemes, but they identify where important sensitivities exist, and thus where further understanding will help to refine OHCA estimates. These results highlight the need for further coordinated OHCA studies to evaluate the performance of existing mapping methods along with comprehensive assessment of uncertainty estimates.
    Description: AS is supported by a Tasmanian Graduate Research Scholarship, a CSIRO-UTAS Quantitative Marine Science top-up, and by the Australian Research Council (ARC) (CE170100023; DP160103130). CMD was partially supported by ARC (FT130101532) and the Natural Environmental Research Council (NE/P019293/1). RC was supported through funding from the Earth Systems and Climate Change Hub of the Australian Government’s National Environmental Science Program. TB is supported by the Climate Observation and Monitoring Program, National Oceanic and Atmosphere Administration, U.S. Department of commerce. GCJ and JML are supported by NOAA Research and the NOAA Ocean Climate Observation Program. This is PMEL contribution number 5065. JAC is supported by the Centre for Southern Hemisphere Oceans Research (CSHOR), jointly funded by the Qingdao National Laboratory for Marine Science and Technology (QNLM, China) and the Commonwealth Scientific and Industrial Research Organization (CSIRO, Australia) and Australian Research Council’s Discovery Project funding scheme (project DP190101173). The research was carried out in part at the Jet Propulsion Laboratory, California Institute of Technology, under a contract with the National Aeronautics and Space Administration (80NM0018D0004). Data used in this study are available on request.
    Keywords: Bias ; Interpolation schemes ; In situ oceanic observations ; Uncertainty ; Oceanic variability ; Trends
    Repository Name: Woods Hole Open Access Server
    Type: Article
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