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  • Articles  (2)
  • American Meteorological Society  (1)
  • Blackwell Science Ltd  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Science Ltd
    Journal of neurochemistry 88 (2004), S. 0 
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The effect of anoxia on Na+/H+ exchange activity was examined in acutely isolated adult rat hippocampal CA1 neurons loaded with the H+-sensitive fluorophore, BCECF. Five-minute anoxia imposed under nominally HCO3–/CO2-free conditions induced a fall in pHi, the magnitude of which was smaller following prolonged exposure to medium in which N-methyl-d-glucamine (NMDG+) was employed as an extracellular Na+ (Na+o) substitute. Also consistent with the possibility that Na+/H+ exchange becomes inhibited soon after the induction of anoxia, rates of Na+o-dependent pHi recovery from internal acid loads imposed during anoxia were slowed, compared to rates of Na+o-dependent pHi recovery observed prior to anoxia. At the time at which rates of pHi recovery were reduced during anoxia, cellular adenosine triphosphate (ATP) levels had fallen to 35% of preanoxic levels, suggesting that ATP depletion might contribute to the observed inhibition of Na+/H+ exchange. In support, incubation of neurons with 2-deoxyglucose and antimycin A under normoxic conditions induced a fall in cellular ATP levels that was also associated with reduced Na+o-dependent rates of pHi recovery from imposed acid loads; conversely, pre-treatment with 10 mm creatine attenuated the effects of anoxia to reduce both ATP levels and Na+o-dependent rates of pHi recovery from internal acid loads. Taken together, the results are consistent with the possibility that functional Na+/H+ exchange activity in adult rat CA1 neurons declines soon after the onset of anoxia, possibly as a result of anoxia-induced falls in intracellular ATP.
    Type of Medium: Electronic Resource
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  • 2
    Publication Date: 2022-09-14
    Description: Author Posting. © American Meteorological Society, 2022. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Journal of Climate 35(2), (2022): 851–875, https://doi.org/10.1175/JCLI-D-20-0603.1.
    Description: The Earth system is accumulating energy due to human-induced activities. More than 90% of this energy has been stored in the ocean as heat since 1970, with ∼60% of that in the upper 700 m. Differences in upper-ocean heat content anomaly (OHCA) estimates, however, exist. Here, we use a dataset protocol for 1970–2008—with six instrumental bias adjustments applied to expendable bathythermograph (XBT) data, and mapped by six research groups—to evaluate the spatiotemporal spread in upper OHCA estimates arising from two choices: 1) those arising from instrumental bias adjustments and 2) those arising from mathematical (i.e., mapping) techniques to interpolate and extrapolate data in space and time. We also examined the effect of a common ocean mask, which reveals that exclusion of shallow seas can reduce global OHCA estimates up to 13%. Spread due to mapping method is largest in the Indian Ocean and in the eddy-rich and frontal regions of all basins. Spread due to XBT bias adjustment is largest in the Pacific Ocean within 30°N–30°S. In both mapping and XBT cases, spread is higher for 1990–2004. Statistically different trends among mapping methods are found not only in the poorly observed Southern Ocean but also in the well-observed northwest Atlantic. Our results cannot determine the best mapping or bias adjustment schemes, but they identify where important sensitivities exist, and thus where further understanding will help to refine OHCA estimates. These results highlight the need for further coordinated OHCA studies to evaluate the performance of existing mapping methods along with comprehensive assessment of uncertainty estimates.
    Description: AS is supported by a Tasmanian Graduate Research Scholarship, a CSIRO-UTAS Quantitative Marine Science top-up, and by the Australian Research Council (ARC) (CE170100023; DP160103130). CMD was partially supported by ARC (FT130101532) and the Natural Environmental Research Council (NE/P019293/1). RC was supported through funding from the Earth Systems and Climate Change Hub of the Australian Government’s National Environmental Science Program. TB is supported by the Climate Observation and Monitoring Program, National Oceanic and Atmosphere Administration, U.S. Department of commerce. GCJ and JML are supported by NOAA Research and the NOAA Ocean Climate Observation Program. This is PMEL contribution number 5065. JAC is supported by the Centre for Southern Hemisphere Oceans Research (CSHOR), jointly funded by the Qingdao National Laboratory for Marine Science and Technology (QNLM, China) and the Commonwealth Scientific and Industrial Research Organization (CSIRO, Australia) and Australian Research Council’s Discovery Project funding scheme (project DP190101173). The research was carried out in part at the Jet Propulsion Laboratory, California Institute of Technology, under a contract with the National Aeronautics and Space Administration (80NM0018D0004). Data used in this study are available on request.
    Keywords: Bias ; Interpolation schemes ; In situ oceanic observations ; Uncertainty ; Oceanic variability ; Trends
    Repository Name: Woods Hole Open Access Server
    Type: Article
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