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  • colon  (5)
  • Springer  (5)
  • American Heart Association (AHA)
  • 1
    Electronic Resource
    Electronic Resource
    Isolation and culture of colonic epithelial cells in serum-free medium (1985)
    Springer
    Methods in cell science 9 (1985), S. 117-122 
    Details
    ISSN: 1573-0603
    Keywords: colon ; epithelial ; serum-free
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Techniques are described for the dissociation, fractionation through Percoll, and in vitro maintenance of mucosal epithelia from the human or guinea pig large bowel. Tissue recovered after surgery is predigested with trypsin-citrate and treated subsequently with a mixture of trypsin, citrate, and collagenase. The resulting suspension of single cells, cell clusters, and partially digested crypts is suspended over a Percoll solution and enriched in multicellular elements by two sequential centrifugations. The recovered multicellular complexes are inoculated to specially treated culture vessels in a serum-free medium supplemented with epidermal growth factor, insulin, transferrin, selenium, and bovine pituitary extract. Epithelia, characterized as such by transmission electron microscopy, adhere to the substrate, form colonies, and can be maintained routinely for study for at least 10 wk.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01797782
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Intracolonic fat inhibits gastric acid secretion independent of gastrin release in the dog (1991)
    Springer
    Digestive diseases and sciences 36 (1991), S. 888-892 
    Details
    ISSN: 1573-2568
    Keywords: enterogastrone ; peptone ; colon ; intragastric titration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The purpose of this study was to examine the effect of perfusion of the colon with a fatty acid (oleic acid) on peptone-stimulated gastric acid secretion and release of gastrin in conscious dogs. Gastric acid secretion was monitored by continuous intragastric titration. Perfusion of the colon with sodium oleate (24 mmol/hr) inhibited gastric acid secretion (14.2±2.6 meq/hr) stimulated by a peptone meal (1%) significantly (P〈0.05) when compared to perfusion of the colon with saline alone (20.1±1.6 meq/hr). The serum elevation, in gastrin in response to intragastric instillation of the peptone meal was not affected by the colonic perfusion of oleic acid. Plasma concentrations of peptide YY (PYY) increased significantly in response to perfusion of the colon with saline or sodium oleate, and the integrated release of PYY in response to sodium oleate 16.9±2.8 ng (60–120) min/ml] was significantly greater than the response to saline [3.1±0.7 ng (60–120) min/ml]. The results of this study indicate that inhibition of gastric acid secretion by perfusion of the colon with fat is not due to an inhibition of gastrin release. In addition, because PYY is an inhibitor of gastric acid secretion, it is possible that PYY participates as an inhibitor of gastric acid secretion by the colon.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01297136
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    The electrophysiology of rabbit descending colon (1982)
    Springer
    The journal of membrane biology 66 (1982), S. 55-61 
    Details
    ISSN: 1432-1424
    Keywords: colon ; apical membrane ; basolateral membrane ; parallel pathways ; current-voltage relations ; electrical circuit model
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary In this paper we employ the data described in the previous paper (I) to derive the current-voltage (I-V) relations of the basolateral membrane, the amiloride-insensitive “leak” pathway across the apical membrane, and the parallel pathways across rabbit descending colon. The results indicate that: a) The resistance of the basolateral membrane is independent of the electrical potential difference across that barrier over the range −8 to 67 mV and averaged 195 Ωcm2. The electromotive force across this barrier averaged 50 mV under control conditions and 48 mV in the presence of amiloride. The origin of this difference is discussed. b) The resistance of the parallel pathways averaged 351 Ωcm2 and was independent of the transepithelial electrical potential difference over the range −170 to +90 mV. The conductance of these pathways can be reasonably well accounted for by the partial ionic conductances of Na, K and Cl reported previously. c) The resistance of the amiloride-insensitive pathway across the apical membrane averaged 1667 Ωcm2 and the electromotive force across this pathway averaged −51 mV. These values are in excellent agreement with those determined by others. The ionic nature of this “leak” pathway remains to be elucidated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01868481
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  • 4
    Electronic Resource
    Electronic Resource
    The electrophysiology of rabbit descending colon (1982)
    Springer
    The journal of membrane biology 66 (1982), S. 41-54 
    Details
    ISSN: 1432-1424
    Keywords: colon ; Na entry ; electrophysiology ; current-voltage relations ; apical membrane ; amiloride
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary A method is described for determining the “instantaneous” transepithelial current-voltage (I-V) relations across rabbit descending colon and deriving theI-V relations of the amiloride-sensitive Na-entry step across the apical membrane. The latter conforms closely to the predictions of the Goldman-Hodgkin-Katz “constant-field” flux equation over a wide range of values of the transapical electrical potential difference (−120 to +50 mV), suggesting that Na entry is the result of simple electrodiffusion through homogeneous pores or channels. The permeability of the apical membrane to Na averaged 0.012 cm/hr, and the intracellular Na activity averaged 10mm. In the studies, the rate of Na entry across the apical membrane varied, spontaneously, over a fourfold range; this variation is entirely attributable to parallel variations in the partial conductance of the apical membrane to Na with no change in the driving force for this movement. Bathing the serosal surface of the tissue with a high-K solution abolishes the electrical potential difference across the basolateral membrane and markedly reduces the resistance of that barrier. Under these conditions, theI-V relations of the amiloride-sensitive Na-entry step across the apical membrane also conform closely to the predictions of the “constant-field” flux equation. Finally, the significance of the point at which the transepithelialI-V relations in the absence and presence of amiloride intersect (“E Na”) and the origin of the “bends” in theseI-V relations at or around this point are discussed. We demonstrate that the point of intersection is simply that value of the transepithelial electrical potential difference at which Na entry is abolished and has no direct bearing on the energetics of the basolateral pump. The “bend” in theI-V relations appears to be due to an increase in the conductance of a pathway in the apical membrane that parallels the Na-entry pathway in the apical membrane that parallels the Na-entry pathway as well as an increase in the conductance of the paracellular pathway; thus, this “bend” does not appear to be directly related to changes in the “active Na transport pathway”.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01868480
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  • 5
    Electronic Resource
    Electronic Resource
    Cyclosporine and α-difluoromethylornithine exhibit differential effects on colon and pancreatic cancer in vitro (1987)
    Springer
    Investigational new drugs 5 (1987), S. 251-258 
    Details
    ISSN: 1573-0646
    Keywords: cancer ; cyclosporine ; pancreas ; colon ; polyamines ; α-difluoromethylornithine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Medicine
    Notes: Abstract α-Difluoromethylornithine (DFMO) is a known irreversible inhibitor of ornithine decarboxylase (ODC), the rate-limiting enzyme in polyamine biosynthesis. Cyclosporine (CsA) has been reported to inhibit ODC activity in vitro. In the present study, we compared the effects of DFMO and CsA on growth, survival, and polyamine levels in mouse colon cancer (MC-26) and hamster pancreatic cancer (H2T) cells in vitro. The growth and survival of MC-26 and H2T cells were inhibited by both DFMO and CsA. However, H2T cells were observed to be significantly more sensitive than MC-26 cells to both CsA and DFMO. The inhibitory effects of CsA were blocked by the addition of the polyamine, putrescine, in both MC-26 and H2T cells. Polyamine levels were altered significantly in both MC-26 and H2T cells treated with CsA and DFMO. However, the profile of these alterations differed between MC-26 and H2T cell lines. Putrescine and spermidine levels in MC-26 cells were more sensitive to DFMO inhibition than were H2T cells. Spermine levels were consistently elevated in MC-26 cells exposed to CsA or DFMO, while the level of spermine in H2T cells decreased significantly in response to the same drugs. These results suggest that CsA and DFMO exhibit different effects on colon and pancreatic cancer growth in vitro. In addition, the differences in the sensitivity of pancreatic and colon cancer to CsA and DFMO indicate potentially important differences in polyamine metabolism between the two cell lines.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00175295
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