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  • American Geophysical Union (AGU)  (1)
  • Blackwell Science Ltd  (1)
  • 1
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background Recent studies have shown that neurotrophins are produced by and can act on several immune-inflammatory cells. The origin of circulating as well as local neurotrophins is unknown.Objectives The aim of this study was to assess whether eosinophils of allergic and non-allergic donors produce, store and release the neurotrophic factors NGF, BDNF and NT-3.Methods Eosinophils were purified by negative immunoselection (purity 〉 96%) from allergic asthmatics and non-allergic donors (25 to 53 years). The presence of mRNA for neurotrophic factors was evaluated by reverse transcription PCR. Specificity was demonstrated by cloning products and sequencing. Stored NGF, BDNF and NT-3 was demonstrated by Western-blotting and flow cytometry. Eosinophils were incubated and supernatants were collected for measurement of neurotrophic factors after cell stimulation with PAF. Neurotrophin content in eosinophil lysates was determined by ELISA.Results Eosinophils demonstrate mRNA for neurotrophins. Proteins were detectable by Western blot and FACS analysis. Neurotrophins were found in the eosinophil lysates at different amounts comparing allergic and non-allergic donors. Cell stimulation with PAF (10-8–10-5 M) after priming with GM-CSF leads to a dose-dependant release of NGF and BDNF.Conclusions Eosinophils store, produce and release NGF, BDNF and NT-3. They are a possible source of elevated neurotrophin levels found in allergy and asthma.
    Type of Medium: Electronic Resource
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  • 2
    Publication Date: 2017-05-19
    Description: ABSTRACT Recent USGS water use report suggests that increasing water-use efficiency could mitigate the supply-and-demand imbalance arising from changing climate and growing population. However, this rich data has not been analyzed to understand the underlying patterns, nor have been investigated to identify the factors contributing to this increased efficiency. A national-scale synthesis of public supply withdrawals (“withdrawals”) reveals a strong North–south gradient in public supply water use with the increasing population in the South contributing to increased withdrawal. Contrastingly, a reverse South–north gradient exists in per-capita withdrawals (“efficiency”), with northern states consistently improving the efficiency, while the southern states’ efficiency declined. Our analyses of spatial patterns of per-capita withdrawals further demonstrate that urban counties exhibit improved efficiency over rural counties. Improved efficiency is also demonstrated over high-income and well-educated counties. Given the potential implications of the findings in developing long-term water conservation measures (i.e., increasing block rates), we argue the need for frequent updates, perhaps monthly to annual, of water use data for identifying effective strategies that control the water-use efficiency in various geographic settings under a changing climate.
    Electronic ISSN: 2328-4277
    Topics: Geosciences
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