GLORIA

GEOMAR Library Ocean Research Information Access

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • Palmarosa  (2)
  • Acampe praemorsa  (1)
  • Springer  (3)
  • American Chemical Society (ACS)
  • 1
    Electronic Resource
    Electronic Resource
    Somatic embryogenesis and plantlet regeneration from cell suspension cultures of palmarosa grass (Cymbopogon martinii) (1997)
    Springer
    Plant cell reports 16 (1997), S. 430-434 
    Details
    ISSN: 1432-203X
    Keywords: Key words Cell suspension culture ; Cymbopogon martinii ; Monocot ; Palmarosa ; Plant regeneration ; Somatic embryogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A cell suspension culture was established from nodal callus of Cymbopogon martinii (Roxb.) Wats in a liquid medium containing Murashige and Skoog (1962) basal salts, vitamins, 100 mg l–1 myo-inositol and 20 g l–1 of sucrose (MS) that was supplemented with 13.6 µM 2,4-dichlorophenoxyacetic acid and 1.15 µM kinetin. An initial inoculum density of 2 × 104 cells ml–1 exhibited optimum cell growth. Calli were obtained 12–15 days after the suspension was plated onto semisolid medium of a similar composition. When calli were transferred to semisolid regeneration medium containing MS + 6.7 µM N 6-benzyl-adenine + 1.15 µM kinetin, somatic embryogenesis and plantlet regeneration occurred after 10–25 days. There was no significant decrease in the regeneration potential of the calli even when the cultures were initiated from 47-week-old cell suspensions. Chromosome counts of cells in suspensions, calli and somatic embryos derived from cultures of different ages revealed the presence of diploids, tetraploids and octaploids. However, the 33 regenerated plants tested were all diploid, indicating that only diploid cells were capable of regeneration in vitro.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01146788
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Somatic embryogenesis and plantlet regeneration from cell suspension cultures of palmarosa grass (Cymbopogon martinii) (1997)
    Springer
    Plant cell reports 16 (1997), S. 430-434 
    Details
    ISSN: 1432-203X
    Keywords: Cell suspension culture ; Cymbopogon martinii ; Monocot ; Palmarosa ; Plant regeneration ; Somatic embryogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A cell suspension culture was established from nodal callus ofCymbopogon martinii (Roxb.) Wats in a liquid medium containing Murashige and Skoog (1962) basal salts, vitamins, 100 mg 1−1 myo-inositol and 20 g l−1 of sucrose (MS) that was supplemented with 13.6 μM 2,4-dichlorophenoxyacetic acid and 1.15 μM kinetin. An initial inoculum density of 2 x 104 cells ml−1exhibited optimum cell growth. Calli were obtained 12–15 days after the suspension was plated onto semisolid medium of a similar composition. When calli were transferred to semisolid regeneration medium containing MS + 6.7 μM N 6-benzyl-adenine + 1.15 μM kinetin, somatic embryogenesis and plantlet regeneration occurred after 10–25 days. There was no significant decrease in the regeneration potential of the calli even when the cultures were initiated from 47-week-old cell suspensions. Chromosome counts of cells in suspensions, calli and somatic embryos derived from cultures of different ages revealed the presence of diploids, tetraploids and octaploids. However, the 33 regenerated plants tested were all diploid, indicating that only diploid cells were capable of regeneration in vitro.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01146788
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Direct shoot regeneration from foliar explants of an epiphytic orchid,Acampe praemorsa (Roxb.) Blatter and McCann (1997)
    Springer
    Plant cell reports 16 (1997), S. 583-586 
    Details
    ISSN: 1432-203X
    Keywords: Acampe praemorsa ; Epiphytic orchid ; In vitro plant regeneration ; Thidiazuron
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An efficient and reproducible procedure is described for the large-scale propagation of an epiphytic orchid,Acampe praemorsa (Roxb.) B latter and McCann using foliar explants. Shoot buds were induced in basal parts of foliar explants on Murashige and Skoog medium supplemented with N6-benzyladenine (BA), kinetin (Kn) or thidiazuron (TDZ), the latter being most effective at 1.0 mg/1. Shoots formed to a TDZ-containing medium elongated following transfer to a substrate supplemented with 2.0 mg/l 1-naphthaleneacetic acid (NAA) and 0.5 mg/1 BA. NAA at lower concentrations had no beneficial effects on shoot regeneration, whether added to the medium along with BA, Kn or TDZ. However, it promoted shoot elongation and leaf expansion. Higher concentrations of NAA suppressed shoot regeneration. The frequency of shoot regeneration was greatly influenced by the developmental stage and orientation of the leaf. Shoots regenerated from the foliar explants were rooted successfully on MS medium containing 1.0 mg/l indole-3-butyric acid. The plantlets were acclimated and eventually transferred to a garden.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01142328
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...