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  • American Association for Cancer Research (AACR)  (6)
  • 1
    Online Resource
    Online Resource
    Abstract 1500: The difference of serum RNA profile: RNA detection method
    American Association for Cancer Research (AACR) ; 2014
    In:  Cancer Research Vol. 74, No. 19_Supplement ( 2014-10-01), p. 1500-1500
    Details
    In: Cancer Research, American Association for Cancer Research (AACR), Vol. 74, No. 19_Supplement ( 2014-10-01), p. 1500-1500
    Abstract: Serum miRNA profiles are widely reported by many research groups, but they do not always coincide well. The reason of this difference is suggested to be, at least in some cases, due to a difference in the RNAdetection method. In this report, we compare the miRNA expression data obtained by different detection tools.. In these days, the whole miRNA expression profiling can be done by multiple qRT-PCR, microarrays, and Next Generation Sequencing. However, the feature of each method will reflect to the obtained data and it is no surprise that data obtained by different tools do not match perfectly. We will compare the serum miRNA expression profiles detected by (1) “TaqMan” Array MicroRNA Card (2) QIAGEN miScript miRNA PCR Array (3) “3D-Gene” Human miRNA oligo chip, (4)”HiSeq” Next Generation Sequencer (5) Nanostring “nCounter” Analysis system and discuss the base of method for serum miRNA profiling. Citation Format: Satoshi Kondo, Satoko Takizawa, Hideo Akiyama. The difference of serum RNA profile: RNA detection method. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 1500. doi:10.1158/1538-7445.AM2014-1500
    Type of Medium: Online Resource
    ISSN: 0008-5472 , 1538-7445
    URL: Article
    DOI: 10.1158/1538-7445.AM2014-1500
    RVK:
    XA 36000
    RVK:
    XA 10000
    Language: English
    Publisher: American Association for Cancer Research (AACR)
    Publication Date: 2014
    detail.hit.zdb_id: 2036785-5
    detail.hit.zdb_id: 1432-1
    detail.hit.zdb_id: 410466-3
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  • 2
    Online Resource
    Online Resource
    Matrix metalloproteinase inhibitor MMI-166 inhibits lymphogenous metastasis in an orthotopically implanted model of lung cancer
    American Association for Cancer Research (AACR) ; 2005
    In:  Molecular Cancer Therapeutics Vol. 4, No. 9 ( 2005-09-01), p. 1409-1416
    Details
    In: Molecular Cancer Therapeutics, American Association for Cancer Research (AACR), Vol. 4, No. 9 ( 2005-09-01), p. 1409-1416
    Abstract: Matrix metalloproteinases (MMP) are considered to be critically involved in tumor invasion and the metastasis of various cancers. MMI-166 is a selective inhibitor of matrix metalloproteinase (MMP-2, MMP-9, and MMP-14). The purpose of this study was to evaluate the effects of MMI-166 on both the growth of the implanted tumor and the lymph node metastasis of the mediastinum and prolonging the life span, using an orthotopic implantation model of the Ma44-3 cancer cell line. We examined the anti-invasive effect of MMI-166 in lung cancer cell lines using an in vitro invasion assay. Next, we examined the anticancer effect of MMI-166 in vivo. MMI-166 (200 mg/kg body weight) or a vehicle was administered orally to the orthotopically implanted lung cancer model. MMI-166 dose-dependently inhibited the invasion of cancer cell lines with expressions of MMP-2 and/or MMP-9 in vitro. In vivo, MMI-166 significantly inhibited mediastinal lymph node metastasis in this orthotopic model (weight of the mediastinum: control, 0.089 ± 0.009 versus MMI-166, 0.069 ± 0.008 mg; P = 0.005; metastatic area: control, 93,495 ± 55,747 versus MMI-166, 22,747 ± 17,478 pixels; P = 0.045). MMI-166 prolonged the life span by 6 days in median survival time in the orthotopically implanted model (P = 0.039). These results showed that MMI-166 could possibly inhibit lymph node metastasis and prolong the life span in lung cancer patients.
    Type of Medium: Online Resource
    ISSN: 1535-7163 , 1538-8514
    URL: Article
    DOI: 10.1158/1535-7163.MCT-05-0031
    Language: English
    Publisher: American Association for Cancer Research (AACR)
    Publication Date: 2005
    detail.hit.zdb_id: 2062135-8
    SSG: 12
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  • 3
    Online Resource
    Online Resource
    Abstract 5294: The difference of serum RNA profile: RNA extraction and detection method.
    American Association for Cancer Research (AACR) ; 2013
    In:  Cancer Research Vol. 73, No. 8_Supplement ( 2013-04-15), p. 5294-5294
    Details
    In: Cancer Research, American Association for Cancer Research (AACR), Vol. 73, No. 8_Supplement ( 2013-04-15), p. 5294-5294
    Abstract: Serum miRNA profiles are widely reported by many .research groups, but they do not always coincide well. The reason of this difference is suggested to be, at least in some cases, due to a difference in the RNA extraction or detection method. In this report, we compare the miRNA expression profiles of RNAs extracted from (1) extracellular vesicles precipitated by ultra-centrifuging (2) precipitation of ExoQuick (3) whole serum by QIAGEN miRNeasy (4) whole serum by Toray "3D-Gene" RNA extraction reagent from liquid sample kit. In addition to this, the comparison of data obtained by different detection tools will be also shown. In these days, the whole miRNA expression profiling can be done by multiple qRT-PCR, microarrays, and Next Generation Sequencing. However, the feature of each method will reflect to the obtained data and it is no surprise that data obtained by different tools do not match perfectly. We will compare the serum miRNA expression profiles detected by (1) “TaqMan” Array MicroRNA Card (2) QIAGEN miScript miRNA PCR Array (3) “3D-Gene” Human miRNA oligo chip, and discuss the base of method for serum miRNA profiling. Citation Format: Satoko Takizawa, Hideo Akiyama, Satoshi Kondo, Nobuyoshi Kosaka, Takahiro Ochiya. The difference of serum RNA profile: RNA extraction and detection method. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 5294. doi:10.1158/1538-7445.AM2013-5294
    Type of Medium: Online Resource
    ISSN: 0008-5472 , 1538-7445
    URL: Article
    DOI: 10.1158/1538-7445.AM2013-5294
    RVK:
    XA 36000
    RVK:
    XA 10000
    Language: English
    Publisher: American Association for Cancer Research (AACR)
    Publication Date: 2013
    detail.hit.zdb_id: 2036785-5
    detail.hit.zdb_id: 1432-1
    detail.hit.zdb_id: 410466-3
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  • 4
    Online Resource
    Online Resource
    Abstract C143: Expression profiles of miRNA, FFPE, and nonamplified samples for diagnostic tests using a supersensitive microarray
    American Association for Cancer Research (AACR) ; 2009
    In:  Molecular Cancer Therapeutics Vol. 8, No. 12_Supplement ( 2009-12-10), p. C143-C143
    Details
    In: Molecular Cancer Therapeutics, American Association for Cancer Research (AACR), Vol. 8, No. 12_Supplement ( 2009-12-10), p. C143-C143
    Abstract: We have developed a novel gene expression microarray system, 3D-Gene™, featuring micro-columnar structure on the platform made of black resin and micro-bead agitation for efficient hybridization. With these technological characteristics, 3D-Gene™ achieves dramatic reduction of background noise, exceptionally high sensitivity and reproducibility, and high correlation with qRT-PCR methodology particularly for genes with low expression. In addition to the general high performance, however, the medical devices in clinical settings must be versatile for a variety of biomarker types as well as sample types. Employing this microarray, we further developed a highly sensitive system to detect attomole-level miRNA which is reported to be a novel biomarker for some cancers. Moreover, we were able to detect gene profiles reproducibly and accurately from formalin-fixed paraffin-embedded (FFPE) tissue which is known to be stored worldwide in a large number for retrospective studies. Finally, we developed a RNA sample preparation protocol with no amplification process, resulting in a quick and cost-effective protocol with high accuracy. Taken together, we developed systems for gene expression detection using a high performance microarray suitable for a wide range of clinical applications. 3D-Gene™ could be a powerful tool for drug target discovery and clinical diagnostic for various diseases. Citation Information: Mol Cancer Ther 2009;8(12 Suppl):C143.
    Type of Medium: Online Resource
    ISSN: 1535-7163 , 1538-8514
    URL: Article
    DOI: 10.1158/1535-7163.TARG-09-C143
    Language: English
    Publisher: American Association for Cancer Research (AACR)
    Publication Date: 2009
    detail.hit.zdb_id: 2062135-8
    SSG: 12
    Location Call Number Limitation Availability
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  • 5
    Online Resource
    Online Resource
    Abstract 5034: Healthy and cancerous serum RNA profiling by the novel RNA extraction reagent and highly sensitive DNA chip
    American Association for Cancer Research (AACR) ; 2012
    In:  Cancer Research Vol. 72, No. 8_Supplement ( 2012-04-15), p. 5034-5034
    Details
    In: Cancer Research, American Association for Cancer Research (AACR), Vol. 72, No. 8_Supplement ( 2012-04-15), p. 5034-5034
    Abstract: Proteins, metabolites and DNA are already known as components of serum or plasma biomarkers, however RNA has not been a strong biomarker candidate because of its instability. Exosomes that are small vesicles secreted by various cells are recently reported to play important roles in intercellular communications by transferring proteins, DNA and also RNA to distant cells through circulatory system. Surprisingly, the exosomal RNA in serum preserves its integrity and thus holds a potential to be a new blood biomarkers. In this report, we show the exhaustive analysis of miRNA and mRNA in serum by DNA chip for the highly purified RNA extracted with a novel reagent. Serum contains various types of nucleic acids, mainly small RNA, mRNA, and also short DNA fragment. We suppose that the contamination of DNA to the extracted RNA often causes a discrepancy between the DNA chip analysis and qRT-PCR validation. The novel reagent was able to extract RNA from serum without contamination of short DNA fragment, resulting in better RNA quantification and decreasing DNA-related noise outputs. Using this novel RNA extraction reagent and the highly sensitive DNA chip “3D-Gene,” we analyzed healthy and cancerous serum miRNA profiles. Over 500 miRNAs are detected in healthy and cancerous sera reproducibly, and some miRNAs were detected specifically in cancerous sera, such as breast, gastric, cervix cancer. In addition, we detected over 19,000 mRNAs from amplified RNA which were similarly extracted from healthy serum and detected by the DNA chip. This indicates that not only serum miRNA but also serum mRNA have a potential capability to be a biomarker. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 5034. doi:1538-7445.AM2012-5034
    Type of Medium: Online Resource
    ISSN: 0008-5472 , 1538-7445
    URL: Article
    DOI: 10.1158/1538-7445.AM2012-5034
    RVK:
    XA 36000
    RVK:
    XA 10000
    Language: English
    Publisher: American Association for Cancer Research (AACR)
    Publication Date: 2012
    detail.hit.zdb_id: 2036785-5
    detail.hit.zdb_id: 1432-1
    detail.hit.zdb_id: 410466-3
    Location Call Number Limitation Availability
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  • 6
    Online Resource
    Online Resource
    Abstract LB-328: Highly sensitive detection of miRNA and mRNA from frozen, FFPE tissue and body fluid samples by expression microarray
    American Association for Cancer Research (AACR) ; 2011
    In:  Cancer Research Vol. 71, No. 8_Supplement ( 2011-04-15), p. LB-328-LB-328
    Details
    In: Cancer Research, American Association for Cancer Research (AACR), Vol. 71, No. 8_Supplement ( 2011-04-15), p. LB-328-LB-328
    Abstract: The RNA profiling from clinical samples readily obtainable in the routine care, such as blood or FFPE samples, is a promising method to find the unknown high-value diagnostic markers. However, as RNA is subjected to degradation even in properly-collected, and immediately frozen tissue, it is more difficult to obtain intact RNA from FFPE or body fluid samples for diagnostic analysis. “3D-Gene” is a highly sensitive gene expression microarray, featuring the unique pillar structure on the substrate and the beads agitation system during the hybridization reaction. Using “3D-Gene”, we achieved highly sensitive detection of mRNA or miRNA from diagnostically important clinical samples. Total RNA was extracted from human serum, plasma and frozen or FFPE tissue samples, with the recommended protocol for each sample. For mRNA detection, total RNA was reverse-transcribed to cDNA and labeled with fluorescent dye directly or after the amplification. For miRNA detection, total RNA was labeled with fluorescent dye directly. These pretreated target nucleotides were hybridized to “3D-Gene” while the hybridized buffer containing target nucleotides was agitated by beads during hybridization. The hybridized microarrays were washed and scanned for image acquisition. (1) With “3D-Gene”, we could detect the mRNA expression profile from FFPE samples with high reproducibility. We also showed high correlation of the expression profiles between FFPE and frozen tissue samples. With 500 ng total RNA obtained from frozen as well as FFPE tissue samples, miRNA was reproducibly detected at attomolar level. Some miRNA biomarkers for various cancers were found from FFPE samples. (2) Serum and plasma are suggested to contain microsomes in which miRNA is enclosed. miRNA from serum and plasma samples were detected with high sensitivity and reproducibility with “3D-Gene”. Some miRNA biomarkers for various cancers were found from patients' serum. “3D-Gene” is a potential tool for detecting transcriptome with high sensitivity and useful for quest and validation of diagnostic biomarkers from clinical samples. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr LB-328. doi:10.1158/1538-7445.AM2011-LB-328
    Type of Medium: Online Resource
    ISSN: 0008-5472 , 1538-7445
    URL: Article
    DOI: 10.1158/1538-7445.AM2011-LB-328
    RVK:
    XA 36000
    RVK:
    XA 10000
    Language: English
    Publisher: American Association for Cancer Research (AACR)
    Publication Date: 2011
    detail.hit.zdb_id: 2036785-5
    detail.hit.zdb_id: 1432-1
    detail.hit.zdb_id: 410466-3
    Location Call Number Limitation Availability
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