In:
Cancer Research, American Association for Cancer Research (AACR), Vol. 75, No. 15_Supplement ( 2015-08-01), p. 5127-5127
Abstract:
Background: Cancer cell culture has contributed with pivotal discoveries for cancer biology and pharmacology. However, cancer cells in culture have not provided a good test method for patient´s response to chemotherapy to a similar extent as antibiograms have been useful to predict response to antibiotics. Although the reason for this is multifactorial, some very common mistakes in cancer cell pharmacology are made in most studies that, if corrected, could improve the overall results. Methods: We systematically tested key conditions such as concentration, time of exposure and analysis, methods of analysis and number of cells analyzed in glioma cell lines. Results: We found that time of exposure to the drug and time of analysis have a large impact on the outcome, with some drugs producing in vitro “relapse” only after 20 days of analysis. Additionally, the initial number of cells plated is also central for the outcome, with resistance being uncovered only after a certain number of cells is treated. Finally, a direct comparison between the MTT viability assay with population doubling assay clearly indicates that the former is not suited for studying effective anticancer drugs and resistance due to its low sensitivity. By systematically analyzing concentration, time of exposure and analysis, number of initial cells and method of analysis we showed that for more meaningful in culture cancer pharmacology, drug exposure should mimic the patient´s exposure time, (5 days for gliomas), analysis should be for at least one treatment cycle (28 days for gliomas), the number of cells tested should be at least more than 15 thousand and the method for analyzing cell number should be repetitive direct counting instead of viability assays such as MTT. Conclusion: Despite not addressing other very fundamental issues in cancer cell culture such as medium, adhesion, 3D culture and presence of other cell types, these simple improvements should aid to produce more significant in culture cancer pharmacology. Financial support: FAPERGS, CNPq e CAPES. Citation Format: Guido Lenz, Andrew O. Silva. The most common mistakes in cancer cell pharmacology. [abstract]. In: Proceedings of the 106th Annual Meeting of the American Association for Cancer Research; 2015 Apr 18-22; Philadelphia, PA. Philadelphia (PA): AACR; Cancer Res 2015;75(15 Suppl):Abstract nr 5127. doi:10.1158/1538-7445.AM2015-5127
Type of Medium:
Online Resource
ISSN:
0008-5472
,
1538-7445
DOI:
10.1158/1538-7445.AM2015-5127
Language:
English
Publisher:
American Association for Cancer Research (AACR)
Publication Date:
2015
detail.hit.zdb_id:
2036785-5
detail.hit.zdb_id:
1432-1
detail.hit.zdb_id:
410466-3
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