In:
The Plant Journal, Wiley, Vol. 47, No. 3 ( 2006-08), p. 457-466
Abstract:
Using the rice PEX14 cDNA as a bait in a yeast two‐hybrid assay, two splice variants of the type I peroxisomal targeting signal (PTS1) receptor, Os Pex5pL and Os Pex5pS, were cloned from a pathogen‐treated rice leaf cDNA library. The proteins were produced from a single gene by alternative splicing, which generated a full‐length variant, OsPEX5L , and a variant that lacked exon 7, OsPEX5S . Os Pex5pL contained 11 copies of the pentapeptide motif WXXXF/Y in its N‐terminus, and seven tetratricopeptide repeats in its C‐terminus. Expression of OsPEX5L and OsPEX5S predominantly occurred in leaf tissues, and was induced by various stresses, such as exposure to the pathogen Magnaporthe grisea , and treatment with fungal elicitor, methyl viologen, NaCl or hydrogen peroxide. The Arabidopsis T‐DNA insertional pex5 mutant, Atpex5 , which does not germinate in the absence of sucrose and was resistant to indole‐3‐butyric acid (IBA), was perfectly rescued by over‐expression of Os Pex5pL, but not by Os Pex5pS. Using transient expression of Os Pex5pL and Os Pex5pS in the Atpex5 mutant, we show that Os Pex5pL translocates both PTS1‐ and PTS2‐containing proteins into the peroxisome by interacting with Os Pex7p, whereas Os Pex5pS is involved only in PTS1‐dependent import in Arabidopsis.
Type of Medium:
Online Resource
ISSN:
0960-7412
,
1365-313X
DOI:
10.1111/tpj.2006.47.issue-3
DOI:
10.1111/j.1365-313X.2006.02797.x
Language:
English
Publisher:
Wiley
Publication Date:
2006
detail.hit.zdb_id:
2020961-7
SSG:
12
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