Description: The purpose of this study was to investigate whether meat from blue mussels (Mytilus edulis) harvested from offshore structures could be used as an alternative protein source in aquaculture fish feed for turbot (Scophthalmus maximus). Two feeding experiments with turbot were conducted in an initial experiment to test the applicability of mussel meal as a fishmeal replacement and/or supplement in turbot feed. In experiment A, the welfare, acceptance, and digestibility of feedstuff with 100%, 50% and 0% of mussel meal replacement for the fishmeal were tested for 8 weeks. In experiment B that followed, the convenience ratio of mussel meal protein in turbot feed for optimum growth was examined. Feedstuff with 25%, 10% and 0% of mussel meal were also fed for 8 (+6) weeks. Growth rates of fish were measured every 2 weeks and their health condition (liver somatic index) determined at the beginning and end of the experiments. Experiment A revealed that a replacement of fishmeal with either 100% or 50% mussel meal resulted in depressed growth (SGR: 1.31 ± 0.04 with 100% mussel meal; 1.54 ± 0.04 with 50% mussel meal; 1.71 ± 0.07 with 0% mussel meal). Experiment B revealed that a fishmeal replacement with 10% or 25% of mussel meal did not reduce growth nor did it have a negative effect on the health of the tested turbot. The study revealed that mussel meal has a high potential to serve as a supplement or as a fishmeal replacement in feed for turbot raised in aquaculture systems. Thus, its use in commercial aquaculture should be given future consideration.

Description: The aim of this study was to determine the macro-parasitic infestation level of oysters from the southern German Bight focussing on copepods of the genus Mytilicola. Crassostrea gigas, Ostrea edulis and Mytilus edulis were collected at five locations: three nearshore sites in the eastern Wadden Sea and two offshore cultivation sites in the German Bight. To reveal seasonal variations one sampling site was investigated in winter and summer. At the nearshore sites, Mytilicola orientalis was regularly detected in C. gigas. Prevalences ranged between 32.3% and 45.1%, intensity between 3.0 ± 0.6 and 8.2 ± 1.5. Infestation rates of C. gigas within the southern German Bight decreased from west to east: Apparently, M. orientalis has started its range extension along the German coast with gradual retardation eastwards but generally followed the invasion route of its main host, the Pacific oyster. Interestingly, we detected not only M. intestinalis but also M. orientalis as an intestinal parasite in M. edulis, which has sofar not previously been described as host within this region. We conclude that M. orientalis is flexible in its host choice. Furthermore, in the eastern Wadden Sea infestation rates of oysters and mussels by copepods are similar. These results deviate from the patterns observed for the northern Wadden Sea in terms of infestation level and host specificity. No macro-parasites were found in oysters and mussels from the offshore sites. This absence can be considered as potentially beneficial for aquaculture activities in the open ocean in terms of stamina and physiological performance.

Description: The purpose of this study was to investigate the effectiveness of 11 different culture media for production of the free-living nematode Turbatrix aceti. Several other harvesting methods were tested in addition to mass production. A further focus was the investigation of amino acid alterations caused by the application of various media during the culture of T. aceti and two additional nematode species, Panagrellus redivivus and Caenorhabditis elegans. Finally, a cost analysis for the production of T. aceti was generated and its outcome compared to the production of conventional live feed organisms. Altogether 11 liquid culture media were tested for mass production of the nematode Turbatrix aceti using a minimum of effort in terms of labour and costs. Six harvesting methods, including filtration as well as active swimming of T. aceti were evaluated. Additional to the culture of T. aceti in four of the above-mentioned media, the nematodes P. redivivus and C. elegans were cultured on two different solid media. Cost analysis for the production of T. aceti includes those of the media, the equipment, as well as the labour costs for culture and harvest. An average density of approx. 30 × 106 ± 8.13 × 106 nematodes L−1 was achieved for T. aceti. The most efficient method (20 μm filtration) allowed harvesting 85.3 ± 2.7% of the nematodes from the medium without disturbing the particles. Lowest efficiency was achieved by combining sedimentation and filtration, accomplishing a harvest of 42.1 ± 5.8%. The amino acid profile of all three nematode species turned out to be both stable and very similar. Amino acid enrichment had little effect. The costs for producing one million T. aceti individuals ranged between 5.39 and 6.19 €, where labour costs accounted for 73 to 84% of the total production costs. In conclusion, T. aceti appears to be very robust, easy to handle, as well as cheaper to cultivate compared to other live-feed organisms. Therefore, its use in commercial aquaculture should be given future consideration.

Description: By incorporating the free-swimming nematode Turbatrix aceti into early feeding regimes of the European whitefish Coregonus maraena, the suitability of this nematode species was investigated as an alternative to Artemia nauplii. During a 14-day feeding trial in a total of 25 aquaria each 1.7 L (each treatment n = 5, 255 larvae/tank) T. aceti was used either as the sole live food or in combination with Artemia nauplii or microdiet to determine the effect of T. aceti on growth performance and survival rate of C. maraena. By analysing the fatty acid composition of T. aceti prior to and after enrichment with INVE spresso® it was investigated whether the amount of n3-polyunsaturated fatty acids (n3-PUFA) in T. aceti could be further enhanced. Supplementation of Artemia nauplii with T. aceti increased growth significantly within the first 5 days of rearing in comparison to the non-supplemented food treatments (14.39 ± 0.15 mm compared to 13.44 ± 0.18 mm; mean ± SE). However, growth and survival of juvenile C. maraena on nematode-supplemented Artemia nauplii did not differ significantly from non-supplemented Artemia nauplii at the end of the 14-day rearing period (15.22 ± 0.15 mm compared to 14.86 ± 0.24 mm). All feeding treatments containing Artemia nauplii showed significantly higher growth and lower mortality at the end of the experiment in comparison to diets containing only the microdiet or T. aceti or a combination thereof. The overall low performance of T. aceti alone can most likely be explained by an insufficient capacity of C. maraena to digest this nematode species efficiently. Enrichment with INVE spresso® successfully increased the proportion of DHA in the T. aceti tissue. The results reveal that T. aceti cannot be considered a full alternative to Artemia nauplii, at least not in the rearing of C. maraena, but might be a useful vector of essential fatty acids within the early rearing period of this and potentially other fish species when provided as live food along with Artemia nauplii.

Description: SummaryThe aim of this study was to identify scientific criteria and to acquire performance data for the development of mariculture in the context of sustainable multifunctional use of offshore space and natural resources.In connection with current plans for a massive expansion of wind farms in offshore areas of the North Sea, the idea emerged to combine these with the installation of extensive mariculture for bivalves and macroalgae. Since offshore wind farms provide safety from shipping and infrastructure for attachment and service support, the opportunity for a multiple-use concept presented itself. However, prior to such a multifunctional development, it is necessary to determine the appropriate biological, technological and management requirements as well as the performance characteristics that would allow the employment of favourable and cost-effective methodologies. To this end, spezial focus was placed on the combination of extensive offshore shellfish and seaweed farming at exposed sites within the proposed offshore wind farms.This thesis deals in detail with1. the effects of abiotic and biotic factors on the growth potential of indigenous macroalgae and bivalves in extensive mariculture systems exposed to the stressful conditions of offshore habitats: (a) effects of physical forces on the cultivated species, (b) various parameters characterising the growth performance of species, (c) testing these parameters while using a variety of substrates and technical setups, (d) survival and health conditions, (e) recruitment, (f) parasite infestation of mussels in relation to inshore and offshore exposure, and (g) site-specific responses of the species to the various harsh offshore environments,2. the interaction between physical offshore processes and man-made structures in areas of the German North Sea Territory where mariculture has potential,3. the conditions for possible combination of mariculture systems with offshore wind farms in terms of the legal framework and possible management strategies, and4. the socio-economic and legal consequences and interactions for offshore mussel farming with the existing conventional inshore bottom culture activities.The research locations were specifically selected sites in the offshore region of the German Bight, mainly in the outer estuary of the River Weser, the offshore waters off the Island of Helgoland, several offshore areas where wind farms are planned, and habitats along the northern part of the Island of Sylt.In this yet relatively young research field the performance of different offshore culture systems with attached macroalgae (Laminaria saccharina) and blue mussels (Mytilus edulis) was assessed at monthly sampling intervals. These procedures were carried out using research vessels, boats and scuba surveys, while determining morphometric parameters (e.g. length, width, thickness) as well as weight for growth analysis of mussels and seaweeds. Furthermore, the condition index, the meat content and the degree of parasite infestation of blue mussels were determined. The abundance of mussel larvae in the water column and the resulting settlement success of post-larvae on various spat collectors were investigated. Seaweed studies included attachment strength, breaking and drag forces on kelp sporophytes in order to describe the resistance of cultivated Laminarians to high energy environments. Additional assessment criteria were biochemical parameters (e.g. chlorophyll-, nutrient-, POC- and TON-concentrations and their ratios) to describe the food availability for mussels and the nutrient composition for algal growth as well as oceanographic parameters (currents, waves, swell, salinity, temperature, light attenuation) to describe the wave climate and current velocities of the study sites in the offshore environment. Technical studies comprised two system designs (longline, ring structure) of various set ups (submerged or floating mode) in different locations (offshore or inshore) to find an appropriate culture design to sustain growth and survival of cultivated species. Further, a specially designed offshore spat collector was used for settlement investigations of mussel post larvae. Some fundamental studies were carried out in order to pave the way for the realisation of open ocean aquaculture in offshore wind farms (e.g. multifunctional use of offshore habitats in terms of avoidance of stakeholder conflicts, the legal framework for such activities in coastal areas and in the Exclusive Economic Zone (EEZ), the multi-use concept in terms of integrated coastal zone management).Laminaria saccharina showed sufficient length increments in offshore habitats growing on a submerged ring system. It could be demonstrated by drag and breaking-/dislodgement force experiments that algae were capable of resisting the high-energy environment of the North Sea. The concentration of mussel larvae in the water column decreased offshore and resulted in a low settlement success while at the same time infestation load decreased and was lacking at offshore suspended collectors. The three system designs showed different results. While the offshore ring proved to be superior for seaweed cultivation under the harsh conditions, the longline showed some major drawbacks in terms of materials used, design and installation modes (submerged or floating). The spat collector largely withstood the harsh hydrodynamic conditions of the North Sea. The hitherto fragmented legal framework for the offshore waters was found to be one of the key constraints for the successful implementation of multifunctional use schemes. However, participation of all involved stakeholders may provide a window of opportunity to turn the existing lack of offshore regulations into a positive momentum, as scope exists to move with the development of new offshore co-management concepts beyond existing management approaches.Whether such offshore installations would provide sufficient economic returns could not be answered yet, as experience on the maintenance of offshore wind farms is lacking so far.However, the discovery that Laminaria saccharina resists offshore forces and shows sufficient length increments and mussels growing in offshore habitats are not affected by parasites may provide a key incentive for positive commercial use.It thus can be shown that there is a high potential for farming the deep blue in the German EEZ, provided that some of the constraints, of which the lack of legal framework and technical aspects seems to be most severe, are resolved in the near future.