In:
European Journal of Microbiology and Immunology, Akademiai Kiado Zrt., ( 2023-09-26)
Abstract:
Mansonellosis is a widely neglected helminth disease which is predominantly observed in tropical regions. This study was conducted to assess potential associations of the prevalence of circulating Mansonella perstans -specific cell-free DNA in human serum and HIV infection in Ghanaian individuals. Methods For this purpose, serum samples obtained from Ghanaian HIV-patients ( n = 989) and non-HIV-infected Ghanaian control individuals ( n = 91) were subjected to real-time PCR targeting the ITS-(internal transcribed spacer-)2 sequence of M . perstans and Mansonella sp. Deux. Results Mansonella -specific cell-free DNA was detected in serum samples of only 2 HIV-positive and 0 HIV-negative individuals, making any reliable conclusions on potential associations between HIV and mansonellosis in tropical Ghana unfeasible. Conclusions Future epidemiological studies on hypothetical associations between mansonellosis and HIV infections should focus more specifically on high-endemicity settings for both Mansonella spp.-infections and HIV-infections, include higher case numbers and be based on real-time PCR from whole blood rather than from serum, in which only circulating parasite DNA but no more cell-bound parasite DNA can be detected. However, the study did not show associations of HIV infections in Ghanaian individuals with Mansonella worm loads high enough to detect cell-free Mansonella DNA in serum by PCR.
Type of Medium:
Online Resource
ISSN:
2062-509X
,
2062-8633
DOI:
10.1556/1886.2023.00028
Language:
Unknown
Publisher:
Akademiai Kiado Zrt.
Publication Date:
2023
detail.hit.zdb_id:
2652327-9
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