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  • AGU (American Geophysical Union)  (7)
  • American Society of Limnology and Oceanography  (1)
  • 1
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    American Society of Limnology and Oceanography
    In:  Limnology and Oceanography: Methods, 15 (9). pp. 753-765.
    Publication Date: 2020-02-06
    Description: Coral epithelia control ion fluxes to the calcification site influencing biomineralization and proxy incorporation. However, data on in vivo characteristics of coral tissue such as permeability, selectivity, and active ion transport are scarce but important for calcification and proxy modeling. To investigate ion permeability and ion fluxes across coral tissues in vivo, we developed an electrophysiological approach for the assessment of active and passive epithelial transport properties. Growing Stylophora pistillata corals in a thin layer over permeable filters allowed ion exchange at the site of skeleton formation for reproducible measurements of electrophysiological properties of coral tissues in a modified Ussing chamber. Compared to former applications, electrical measurements on these coral filter units were dominated by tissue characteristics with minimal influence of skeleton or physical stress. Coral tissues were cation selective. Their overall high electrical resistance characterized them as tight epithelia indicating low paracellular permeability for passive ion diffusion. This includes ions relevant for calcification. A small short-circuit current indicates active charge transport across the entire coral tissue. The present approach is applicable to corals laterally overgrowing substrates. It allows the electrophysiological characterization of coral tissue in vivo in response to environmental conditions. This will improve our knowledge on transepithelial transport relevant for biomineralization in corals.
    Type: Article , PeerReviewed
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  • 2
    Publication Date: 2019-09-23
    Description: Mytilus edulis were cultured for 3 months under six different seawater pCO(2) levels ranging from 380 to 4000 mu atm. Specimen were taken from Kiel Fjord (Western Baltic Sea, Germany) which is a habitat with high and variable seawater pCO(2) and related shifts in carbonate system speciation (e. g., low pH and low CaCO3 saturation state). Hemolymph (HL) and extrapallial fluid (EPF) samples were analyzed for pH and total dissolved inorganic carbon (C-T) to calculate pCO(2) and [HCO3-]. A second experiment was conducted for 2 months with three different pCO(2) levels (380, 1400 and 4000 mu atm). Boron isotopes (delta B-11) were investigated by LA-MC-ICP-MS (Laser Ablation-Multicollector-Inductively Coupled Plasma-Mass Spectrometry) in shell portions precipitated during experimental treatment time. Additionally, elemental ratios (B/Ca, Mg/Ca and Sr/Ca) in the EPF of specimen from the second experiment were measured via ICP-OES (Inductively Coupled Plasma-Optical Emission Spectrometry). Extracellular pH was not significantly different in HL and EPF but systematically lower than ambient water pH. This is due to high extracellular pCO(2) values, a prerequisite for metabolic CO2 excretion. No accumulation of extracellular [HCO3-] was measured. Elemental ratios (B/Ca, Mg/Ca and Sr/Ca) in the EPF increased slightly with pH which is in accordance with increasing growth and calcification rates at higher seawater pH values. Boron isotope ratios were highly variable between different individuals but also within single shells. This corresponds to a high individual variability in fluid B/Ca ratios and may be due to high boron concentrations in the organic parts of the shell. The mean delta B-11 value shows no trend with pH but appears to represent internal pH (EPF) rather than ambient water pH.
    Type: Article , PeerReviewed
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  • 3
    Publication Date: 2018-02-28
    Description: Modern analogs are an essential part of palaeoclimate studies, because they provide the basis for the understanding of geochemical signatures of fossils. Ostreoids are common in many sedimentary sequences and because of their fast growth, high temporal resolution sampling of past seasonal variability is possible. Here, two shell structures of modern Giant Pacific Oysters (Crassostrea gigas), the chalky substance and foliate layers, have been sampled for trace element distributions (Mg, Sr, Mn) and stable isotope variability (C, O, Ca). Oxygen isotopes exhibit a clear seasonal signature. Mean carbon isotope values of different oysters agree within 0.1‰, but ontogenic variability is complicated by shell growth patterns and potential small vital effects. The calcium isotope ratios are found to be constant throughout ontogeny within analytical precision at a value of δ44/40Ca = 0.68 ± 0.16‰ (2 sd) SRM–915a which is consistent with other bivalve species. Calcium isotope ratios in oyster shell material might thus be a possible proxy for palaeo seawater calcium isotope ratios. Element/Ca ratios are significantly higher in the chalky substance than in the foliate layers and especially high Sr/Ca and Mn/Ca ratios are observed for the first growth season of the oysters. Mg/Ca ratios in the chalky substance show a negative correlation with δ18O values, compatible with a temperature dependence, whereas this correlation is absent in the foliate layers. Seasonal changes of Sr/Ca are controlled by metabolic processes, whereas for Mn/Ca an additional environmental control is evident.
    Type: Article , PeerReviewed
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  • 4
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    AGU (American Geophysical Union)
    In:  Eos, Transactions American Geophysical Union, 79 (52). pp. 633-636.
    Publication Date: 2017-02-10
    Description: The potential for using sclerosponges, marine organisms that secrete a hard calcerous skeleton, as paleoclimatic indicators has attracted the interest of a number of scientists. Sclerosponges are composed mainly of calcium carbonate and they are very long lived. Variations in their skeletal chemistry contain proxy information regarding their environment and that information has the potential to augment, if not supplant, data from scleractinian corals in interpreting past water temperature, salinity, and productivity over periods of 100s to 1000s of years. Sclerosponges, or calcified demosponges, contain aragonite or calcite and a small amount of siliceous material. Lang et al. [1975] report that these sponges grow within a reef framework, under coral talus in the shallower parts of a reef less than 55 m deep and on steep surfaces of the fore-reef between 55 and 145 m deep. The largest and most conspicuous of the sclerosponges described by those authors is Ceratoporella nicholsoni (Figure 1), which is reported to attain a diameter in excess of 1 m. These sponges are similar in growth habit to many massive vanities of scleractinian corals, the live sponge inhabiting the upper portion of the skeleton, while the lower portion of the skeleton is essentially dead.
    Type: Article , NonPeerReviewed
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  • 5
    Publication Date: 2018-01-30
    Description: Coralline sponge skeletons are excellent tools for reconstructing the carbon isotope history ofdissolved inorganic carbon (DIC) in tropical surface waters. Carbon isotope records from corallinesponges clearly reflect the industrial12C increase in atmospheric CO2with a precision that permitsquantitative interpretations. We find from a set of d13C records of four Caribbean sponge specimensthat the isotopic response of surface water DIC to the changing isotopic composition of atmosphericCO2varied dynamically during the last century, depending on the rate of atmospheric change. Three ofour sponges provide 600 year long d13C records. For the first time, we can reconstruct surface waterd13CDICfor the full history of the industrial CO2release as well as the preceding preindustrial periodback to the beginning of the Little Ice Age. This provides a well-founded estimate of theanthropogenically uninfluenced, preindustrial background level of surface water13C/12C ratios. Ourrecords show small but systematic variations that appear to be linked to the climate fluctuations of theLittle Ice Age.
    Type: Article , PeerReviewed
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  • 6
    Publication Date: 2017-05-10
    Description: We investigate aragonitic skeletons of the Caribbean sclerosponge Ceratoporella nicholsoni from Jamaica, 20 m below sea level (mbsl), and Pedro Bank, 125 mbsl. We use d18O and Sr/Ca ratios as temperature proxies to reconstruct the Caribbean mixed layer and thermocline temperature history since 1400 A.D. with a decadal time resolution. Our age models are based on U/Th dating and locating of the radiocarbon bomb spike. The modern temperature difference between the two sites is used to tentatively calibrate the C. nicholsoni Sr/Ca thermometer. The resulting calibration points to a temperature sensitivity of Sr/Ca in C. nicholsoni aragonite of about -0.1 mmol/mol/K. Our Sr/Ca records reveal a pronounced warming from the early 19th to the late 20th century, both at 20 and 125 mbsl. Two temperature minima in the shallow water record during the late 17th and early 19th century correspond to the Maunder and Dalton sunspot minima, respectively. Another major cooling occurred in the late 16th century and is not correlatable with a sunspot minimum. The temperature contrast between the two sites decreased from the 14th century to a minimum in the late 17th century and subsequently increased to modern values in the early 19th century. This is interpreted as a long-term deepening and subsequent shoaling of the Caribbean thermocline. The major trends of the Sr/Ca records are reproduced in both specimens but hardly reflected in the d18O records.
    Type: Article , PeerReviewed
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  • 7
    Publication Date: 2019-09-23
    Description: We reconstruct Caribbean seawater temperatures from sclerosponge Sr/Ca ratios using a specimen of Ceratoporella nicholsoni that grew at 20 m below sea level in a reef cave at Jamaica. We sample the time interval from 1620 to 1745 A.D. with almost monthly resolution. This interval includes the Maunder sunspot minimum, one of the coldest periods of the Little Ice Age. Reconstructed annual temperature amplitudes are on the order of about 1°C. The mean growth rate calculated from the annual Sr/Ca variations corresponds perfectly with U-Th-based growth rates. We find that the interannual climate variability is determined by El Niño–Southern Oscillation and by a decadal signal, most likely originating from the tropical North Atlantic. On a multidecadal timescale the Maunder Minimum is characterized by a 1°–2°C cooling and reduced amplitudes of the interannual and decadal temperature variations.
    Type: Article , PeerReviewed
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  • 8
    Publication Date: 2019-09-23
    Description: Measurements of the calcium isotopic composition (δ44/40Ca) of planktonic foraminifera from the western equatorial Pacific and the Indian sector of the Southern Ocean show variations of about 0.6‰ over the past 24 Myr. The stacked δ44/40Ca record of Globigerinoides trilobus and Globigerina bulloides indicates a minimum in δ44/40Casw (seawater calcium) at 15 to 16 Ma and a subsequent general increase toward the present, interrupted by a second minimum at 3 to 5 Ma. Applying a coupled calcium/carbon cycle model, we find two scenarios that can explain a large portion of the observed δ44/40Casw variations. In both cases, variations in the Ca input flux to the ocean without proportional changes in the carbonate flux are invoked. The first scenario increases the riverine calcium input to the ocean without a proportional increase of the carbonate flux. The second scenario generates an additional calcium flux from the exchange of Ca by Mg during dolomitization. In both cases the calcium flux variations lead to drastic changes in the seawater Ca concentrations on million year timescales. Our δ44/40Casw record therefore indicates that the global calcium cycle may be much more dynamic than previously assumed.
    Type: Article , PeerReviewed
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