In:
Acta Endocrinologica, Oxford University Press (OUP), Vol. 42, No. 4_Suppl ( 1963-04), p. S3-S34
Abstract:
The separation and the identification of steroids in biological fluids by gas phase chromatography are described. Comparison of hydrolytic procedures indicated that fractionation into groups of similar compounds: pregnanediol, 17-ketosteroids, estrogens and corticoids was necessary. Bongiovanni's method was qualitatively and quantitatively superior for urinary pregnanediol in crude and purified extracts. Enzymatic hydrolysis, solvolysis and acid hydrolysis with and without simultaneous extraction were employed for the commonly occurring 11-oxo- and desoxy-17-ketosteroids and the estrogens. Solvolysis yielded the highest concentration of DHEA and seemed fairly specific for this steroid. Estrone, estradiol and estriol have been separated and identified as their acetoxy derivatives. Cortisone, cortisol and their tetrahydro reduced forms were detected in extracts from urines hydrolyzed with beta-glucuronidase and with acid at pH 1.0 at room temperature. Column and paper chromatography were used for purifying the extracts but molecular alteration was observed with cortisol in extracts from papergrams which had been scanned by ultraviolet light. The order of the migration of steroids was reversed from that usually observed on paper chromatograms.
Type of Medium:
Online Resource
ISSN:
0804-4643
,
1479-683X
DOI:
10.1530/acta.0.042S000003
Language:
Unknown
Publisher:
Oxford University Press (OUP)
Publication Date:
1963
detail.hit.zdb_id:
1183856-5
detail.hit.zdb_id:
1485160-X
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