In:
Journal of Bacteriology, American Society for Microbiology, Vol. 100, No. 3 ( 1969-12), p. 1364-1370
Abstract:
The decay of alkaline phosphatase messenger ribonucleic acid (mRNA) in Escherichia coli was studied in cells sensitized to actinomycin D by ethylenediaminetetra-acetate treatment. It was found that in the wild-type strain (K-10) as well as in a nonsense mutant (S26C200), which is phenotypically reversible by treatment with 5-fluorouracil, mRNA decays with a half-life of 2.0 to 2.5 min. Similarly, in a 3-min pulse of labeled 5-fluorouracil, 70% of the incorporated analogue is contained in the labile RNA fraction decomposing with a half-life of 1.8 min.
Type of Medium:
Online Resource
ISSN:
0021-9193
,
1098-5530
DOI:
10.1128/jb.100.3.1364-1370.1969
Language:
English
Publisher:
American Society for Microbiology
Publication Date:
1969
detail.hit.zdb_id:
1481988-0
SSG:
12
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