In:
Applied Microbiology, American Society for Microbiology, Vol. 18, No. 3 ( 1969-09), p. 433-437
Abstract:
Hemagglutinating and complement-fixing antigens of La Crosse virus (California arbovirus group) were produced in serum-free suspension cultures of BHK-21/13S cells. The appearance and production of these antigens were correlated with the titer of infectious virus. No significant differences in antigen titers were produced by varying virus dose 10-fold. Hemagglutinin appeared 6 to 8 hr after inoculation and reached peak titer in 14 to 22 hr. Both β-propiolactone and Tween 80-ether treatment inactivated infectious virus in the antigens. Unlyophilized antigen was stable at -60, 5 and 24 C for at least 117 days but not for 1 year. Lyophilized antigen was stable for at least a year, however, at -20 and 5 C. Cell culture-produced antigen was more sensitive than brain-produced antigen in detecting hemagglutination inhibition antibody in human sera.
Type of Medium:
Online Resource
ISSN:
0003-6919
DOI:
10.1128/am.18.3.433-437.1969
Language:
English
Publisher:
American Society for Microbiology
Publication Date:
1969
detail.hit.zdb_id:
207801-6
detail.hit.zdb_id:
1478346-0
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