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  • 1970-1974  (4)
Keywords
Language
Years
Year
  • 1
    Map
    Map
    London : Longman
    Keywords: Atlas ; Pilze ; Ultrastruktur
    Type of Medium: Map
    Pages: 221 S , zahlr. Ill , 29 cm
    Edition: 1. publ.
    ISBN: 058244134X
    DDC: 589/.2/0440222
    RVK:
    RVK:
    Language: English
    Note: Includes bibliographies and indexes
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 17 (1970), S. 0 
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SYNOPSIS. Cultures of Telotrochidium henneguyi, begun with logarithmic phase cells, were employed in an effort to produce synchronized fission by heat treatment. The cells tolerated a temperature range of 20–50 C; temperatures above 50 were lethal. When cells were exposed to a single shock for 30 min, 30–40 produced 0–50% encystment with total excystment after 10 min exposure to room temperature (heat shock range). No encystment occurred between 20–30 (intershock range). Encystment and excystment time varied directly with temperature between 40–50.The most effective procedure for inducing synchronized fission consisted of 6 cycle program of 38/28 C (shock temperature/intershock temperature) administered for 15/15 (shock/intershock duration in min). Division indices (DI = cells dividing/total population X 100 =%) ranged from 12–66% with a mean of 37.25%. In control cells, division indices ranged from 2–20% with an average of 12%. Inferences from these independently derived findings are discussed.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 21 (1974), S. 0 
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: A synthetic medium for Opercularia coarctata was developed that contains 20 amino acids, 10 vitamins, an 8-component balanced salt solution, Fe2(SO4)3·(NH4)2SO4·24H2O, Tween 80, stigmasterol, a 7-component nucleic acid mixture, phenol red as an indicator, and 2,500 U.S.P. units/ml penicillin to maintain sterility. This medium supported axenic survival for 96 hr. Multiple supplements of thioctic acid, niacin, niacinamide, inositol, PABA, oleic acid, and Fe(NO3)2·9H2O instead of Fe2(SO4)3·(NH4)2SO4·24H2O coverted the survival medium into a growth medium, which permitted 36–45 days continuous cultivation of populations in excess of 4 × 103 cells/3.0 ml final volume. Five generations were produced during the 48 hr logarithmic growth period. Serial transfers at 72 hr and during periods of greatest cell density produced a maximum of 8 generations 96 hr after initiation but the medium failed to sustain growth through more than 6 serial transfers. Extension of this investigation to formulating a minimal axenic medium is discussed.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Protoplasma 82 (1974), S. 341-364 
    ISSN: 1615-6102
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The role and distribution of the Golgi apparatus has been compared in hymenial and subhymenial cells ofCoprinus cinereus using conventional electron microscopic and carbohydrate localization techniques. Basidia at early interphase II of meiosis possessed numerous single Golgi cisternae. Golgi vesicles may contain fibrous granular material similar to basidial and basidiospore walls. Vesicles similar in size and apparently in content to those on cisternae accumulated at the growing apex of the young basidiospore. Golgi vesicles were also found in cystidia but appeared to be absent in other cells studied. Pseudoparaphyses, cystidia and subhymenial cells contained large deposits of glycogen which were removed enzymatically in light microscope preparations, but carbohydrate staining persisted in the cytoplasm of basidia, cystidia and basidiospores at the probably sites of Golgi vesicles and cisternae after enzymatic digestion. Septal pore caps of subhymenial cells were surrounded by a fibrillar cytoplasmic zone devoid of cell organelles except ribosomes. The periodic acid-silver hexamine and silver protein techniques for ultrastructural localization of carbohydrates were compared; the latter gave specific results with the controls used. Carbohydrates were localized in certain wall layers of the immature basidiospore and in the contents of Golgi vesicles. Staining also occurred in glycogen, plasma membrane and lomasomes. In the septal pore apparatus staining occurred only in the septal wall and a region in the septal pore swelling probably containing fibrils. The wall of the pseudoparaphyses stained more than the basidial wall. The results suggest that carbohydrates accumulate in Golgi vesicles attached to cisternae, that changes in vesicle contents occur during migration to the basidiospore, and that these vesicles may transport polysaccharides and mucopolysaccharides to the developing basidiospore wall.
    Type of Medium: Electronic Resource
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