ISSN:
1432-8798
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary The mengovirus-induced 250 S RNA polymerase structure was separated from the bulk of the soluble protein and membrane particulates by two cycles of sucrose gradient centrifugation. After labeling cells 2–5 hours after infection the major polypeptides detected were capsid polypeptides α and γ and polypeptides F and G. Intermediate amounts of capsid precursor s and capsid polypeptide β were also present. Small amounts of capsid precursors A, B, and D1 and stable polypeptide E were also detected. About 18–20 per cent of the labeled protein associated with the 250 S polymerase structure was present in mengovirus particles which cosediment with the polymerase preparation. After removal of these virus particles by CsCl gradient centrifugation the RNA-associated proteins recovered in the CsCl gradient pellet contained polypeptides ɛ, α, and γ, capsid precursors A and B and the stable polypeptides E, F, and G. The capsid polypeptide β is sharply reduced in amount and the reduction can be accounted for the by percent of mengovirus contaminating the polymerase structure. This polypeptide composition resembles the composition of poliovirus procapsid polypeptides in that it contains precursor capsid polypeptides and a low amount of capsid polypeptides β.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01240068
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