In:
Journal of Bacteriology, American Society for Microbiology, Vol. 101, No. 1 ( 1970-01), p. 292-296
Kurzfassung:
A nitrogenase system that remains in the supernatant fluid after centrifuging for 3 hr at 180,000 × g can be extracted from Azotobacter vinelandii by osmotic lysis of the bacteria. This nitrogenase preparation is oxygen-labile and appears to be similar, though not identical, to that obtained from Clostridium pasteurianum . The particulate characteristic and oxygen stability of previously described preparations are likely due to the method of cell disruption, e.g., in the French pressure cell. The data support a nitrogenase model system in the intact cell in which oxygen-labile enzymes are protected from oxygen by the extensive internal membranous system which Azotobacter synthesize only when they fix nitrogen.
Materialart:
Online-Ressource
ISSN:
0021-9193
,
1098-5530
DOI:
10.1128/jb.101.1.292-296.1970
Sprache:
Englisch
Verlag:
American Society for Microbiology
Publikationsdatum:
1970
ZDB Id:
1481988-0
SSG:
12
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