In:
Proceedings of the National Academy of Sciences, Proceedings of the National Academy of Sciences, Vol. 70, No. 8 ( 1973-08), p. 2266-2270
Kurzfassung:
Earlier electrophoretic analyses have shown that the 60-70S RNA of avian sarcoma viruses contains a characteristic subunit, termed class a subunit, which has a lower electrophoretic mobility than class b subunit found in transformation-defective derivatives of sarcoma viruses and in avian leukosis viruses. We have compared the RNAs of three nondefective avian sarcoma viruses, B77 and Prague and Schmidt-Ruppin strains of Rous sarcoma virus, with those of their transformation-defective ( td ) derivatives, td B77, td PR-C, and td SR-A, respectively, to determine the chemical basis for the difference between class a and b subunits. It was found by “fingerprinting” that ( 1 ) all (about 20-25) large T1 RNase-resistant oligonucleotides present in class b subunits of transformation-defective viruses have homologous counterparts in the class a subunits of corresponding nondefective sarcoma viruses and that ( 2 ) class a subunits contain a few (one or two) additional oligonucleotides that are not present in class b . By contrast the oligonucleotide fingerprints of avian tumor viruses of different strains and subgroups were very different. Cross hybridization of classes a and b RNA of sarcoma virus B77 with DNA transcribed from a corresponding transformation-defective virus td B77 showed that the two RNAs share at least 60% and differ by about 10% of their sequences. It is suggested that the structural relationship of class a and b subunits of corresponding viruses may be expressed as a = b + x , and that all the oligonucleotides present only in RNAs of sarcoma viruses but not in transformation-defective viruses of the corresponding strains are part of sequence(s) x . The possibility that x represents genetic information directly or indirectly involved in transformation of fibroblasts is discussed.
Materialart:
Online-Ressource
ISSN:
0027-8424
,
1091-6490
DOI:
10.1073/pnas.70.8.2266
Sprache:
Englisch
Verlag:
Proceedings of the National Academy of Sciences
Publikationsdatum:
1973
ZDB Id:
209104-5
ZDB Id:
1461794-8
SSG:
11
SSG:
12
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