In:
The Journal of Immunology, The American Association of Immunologists, Vol. 113, No. 3 ( 1974-09-01), p. 876-884
Abstract:
Recombinant IgG molecules possessing the heavy chain markers transmitted in repulsion were isolated from a rabbit serum and analyzed. The rabbit genotype was a1/a2; e14/e15, a1 being in coupling with e14 and a2 with e15. Recombinant molecules carrying either a1 and e15 or a2 and e14 were obtained in a relatively pure form by sequential absorption and elution with cross-linked antisera. a1–e15 molecules also were compared by immunoelectrophoresis with other IgG fractions, “nonrecombinant” IgG and “a-minus” IgG, isolated from the same serum. All fractions showed similar immunoelectrophoretic patterns. a1–e15 recombinant molecules were found to have a normal κ:λ light chain ratio and a normal κ-chain allotypes ratio. Furthermore, they could be dissociated into heavy and light chains which elute after gel filtration at the same positions as nonrecombinant molecules.
Type of Medium:
Online Resource
ISSN:
0022-1767
,
1550-6606
DOI:
10.4049/jimmunol.113.3.876
Language:
English
Publisher:
The American Association of Immunologists
Publication Date:
1974
detail.hit.zdb_id:
1475085-5
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