In:
Proceedings of the National Academy of Sciences, Proceedings of the National Academy of Sciences, Vol. 66, No. 4 ( 1970-08), p. 1260-1266
Abstract:
The Escherichia coli tRNA Tyr gene carried by the ϕ80psu 3 + transducing phage was transcribed in vitro by DNA-dependent RNA polymerase. The enzymatically synthesized tRNA Tyr -like polynucleotide chains were detected by competition with purified E. coli 32 P-tRNA Tyr for specific hybridization sites on ϕ80psu 3 + DNA. Analysis by sucrose gradient centrifugation showed the tRNA Tyr -like chains to possess a heterogeneous distribution with respect to sedimentation coefficients, with a broad peak around 8 S. The presence of the termination factor ρ during the transcription did not significantly reduce the average size of the in vitro synthesized tRNA Tyr -like chains.
Type of Medium:
Online Resource
ISSN:
0027-8424
,
1091-6490
DOI:
10.1073/pnas.66.4.1260
Language:
English
Publisher:
Proceedings of the National Academy of Sciences
Publication Date:
1970
detail.hit.zdb_id:
209104-5
detail.hit.zdb_id:
1461794-8
SSG:
11
SSG:
12
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