In:
Proceedings of the National Academy of Sciences, Proceedings of the National Academy of Sciences, Vol. 67, No. 1 ( 1970-09), p. 68-73
Abstract:
T4 polynucleotide ligase efficiently catalyzes the head-to-tail joining of the ribo-oligoadenylates, r-(pA) 8 and r-(pA) 10 , in the presence of high molecular weight deoxypolythymidylate. The enzyme also catalyzes the joining of deoxy-oligothymidylates, e.g., d-(pT) 10 , in the presence of ribopolyadenylate. The enzyme failed to bring about the joining of r-(pA) 10 when poly r-U was used as the template, although a slow formation of the expected activated intermediate from r-(pA) 10 was detected.
Type of Medium:
Online Resource
ISSN:
0027-8424
,
1091-6490
DOI:
10.1073/pnas.67.1.68
Language:
English
Publisher:
Proceedings of the National Academy of Sciences
Publication Date:
1970
detail.hit.zdb_id:
209104-5
detail.hit.zdb_id:
1461794-8
SSG:
11
SSG:
12
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