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  • 1
    Online Resource
    Online Resource
    Bacterial, Phage and Molecular Genetics : An Experimental Course. (1976)
    Berlin, Heidelberg :Springer Berlin / Heidelberg,
    Details
    Keywords: Bacterial genetics-Experiments. ; Viral genetics-Experiments. ; Molecular genetics-Experiments. ; Electronic books.
    Type of Medium: Online Resource
    Pages: 1 online resource (243 pages)
    Edition: 1st ed.
    ISBN: 9783642663147
    URL: https://ebookcentral.proquest.com/lib/geomar/detail.action?docID=6698647
    DDC: 576.1
    Language: English
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    GEOMAR EBL
  • 2
    Electronic Resource
    Electronic Resource
    Pleiotropic consequences of mutations towards antibiotic-hypersensitivity in Serratia marcescens (1978)
    Springer
    Archives of microbiology 116 (1978), S. 259-268 
    Details
    ISSN: 1432-072X
    Keywords: Serratia marcescens ; Antibiotics, hypersensitivity to ; Membranes, bacterial ; Enzymes, extracellular ; Lipase, extracellular ; Mutation, pleiotropic ; Proteins, outer membrane ; Detergents, lysis by ; Pectin ; Glycogen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Various mutants (oxa s ) were isolated from Serratia marcescens SM-6 by selecting for hypersensitivity towards oxacillin. All mutants found are highly pleiotropic and able to yield spontaneous revertants which behave like the wild-type. Mutant W 1421 mostly studied shows the following phenotypic properties not found in the wild-type: (1) The growth is hypersensitive to various antibiotics, detergents and dyes which differ remarkably in their chemical structure and antibacterial action-mechanism, (2) the cells can be easily solubilized by 0.05% Sodium-dodecylsulfate, (3) the cells allow the adsorption of the roughmutant specific Salmonella phage 6SR, (4) strong cellular binding of crystal violet, (5) agglutination of the cells in 0.3% auramin solution and (6) reduced formation of red pigment. Strain W 1421 is assumed to be a lipopolysaccharide-defective mutant. The outer membrane of mutant W 1421 analyzed by Sodiumdodecylsulfate-polyacrylamide gel electrophoresis possesses a single protein less than that of the wild-type. Mutant W 1421 is further characterized by its low exolipase activity; exoprotease and exonuclease activities are as in the wild-type. This specific exoenzyme deficiency can be overcome either by backmutation to oxacillin-resistance or by growing mutant W 1421 in a medium supplemented with certain non-metabolizable polysaccharides, e.g. glycogen or pectin B. Both polysaccharides increase the exolipase activity of the wild-type too.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00417849
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Mutants of Serratia marcescens lacking cyclic nucleotide phosphodiesterase activity and requiring cyclic 3′,5′-AMP for the utilization of various carbohydrates (1975)
    Springer
    Archives of microbiology 104 (1975), S. 189-196 
    Details
    ISSN: 1432-072X
    Keywords: Phosphodiesterase ; Adenylate Cyclase ; Cyclic AMP ; Serratia marcescens
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Adenine requiring mutants of Serratia marcescens SM-6-F'lac + have been found to grow well in minimal-glucose medium solely supplemented with cAMP. From one of these ade strains double mutants (called ade cpd) were isolated which could no longer utilize cAMP but which still grew on 5′AMP. Dialyzed cell extracts (soluble fraction) of the double mutants, assayed for cAMP phosphodiesterase, were unable to hydrolyze cAMP whereas cell extracts of the parental strains yielded 5′AMP at a rate of 1.6–2.0 μmoles min−1 mg−1 protein. The loss of the phosphodiesterase activity in S. marcescens cpd W1181 did not cause an accumulation of large amounts of cAMP as was found for the diesterase-negative mutant AB257pc-1 of Escherichia coli. The induced synthesis of β-galactosidase in mutant cpd W 1181 showed about the same sensitivity to transient and permanent catabolite (glucose) repression as the corresponding cpd + strain. Starting from S. marcescens cpd W1181 three independent double mutants (called cpd cya) were isolated which required exogenous cAMP for utilizing various carbohydrates as carbon source, for motility and for the formation of extracellular lipase and the red pigment prodigiosine. The intracellular concentration of cAMP in these mutants, grown in nutrient broth, was 40–60% of that of the parental strain which is about 4×10−4 M. However, the adenylate cyclase in cell extracts of the mutants W1237 and W1270 was like that of the corresponding cya + strain (about 2×10−2 μmoles min−1 mg−1 protein).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00447323
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Relaxed mutants ofSerratia marcescens SM-6. Biochemical traits and relevance of therel + allele for the formation of exoenzymes (1979)
    Springer
    Archives of microbiology 121 (1979), S. 181-186 
    Details
    ISSN: 1432-072X
    Keywords: Enzymes, extracellular ; Escherichia coli ; Guanosine tetra-pentaphosphate ; Lipase, extracellular ; Nuclease, extracellular ; Protease, extracellular ; Regulation of RNA synthesis ; Regulation of exoenzyme formation ; RNA-control ; Stringent and relaxed ; Serratia marcescens
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Serratia marcescens SM-6 when starved for a required amino acid stops synthesizing protein and RNA and accumulates two nucleotides which cochromatograph with ppGpp and pppGpp. These features are characteristic of bacterial strains with stringent RNA control (rel +). Two independent mutants were isolated which resemble relaxed (relA) mutants ofEscherichia coli; they continue to synthesize RNA and accumulate neither ppGpp nor pppGpp when deprived of the required amino acid. The extracellular enzyme activities (nuclease, protease, lipase) of the relaxed mutants are about the same as those of the parental stringent strain when studied under standard growth conditions. Exoenzyme-deficient (nuc; prt) and exoenzyme-hyperproducing (nuc su) mutants were isolated from both stringent and relaxed strains ofS. marcences SM-6 and no change of the cellular ability to form ppGpp and pppGpp could be observed. From these results it appears that the formation of exoenzymes ofS. marcescens SM-6 is independent of stringent/relaxed RNA control.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00689984
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    Paper (German National Licenses)
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