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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 120 (1979), S. 47-51 
    ISSN: 1432-072X
    Keywords: Campylobacter ; Anaerobic electron transport ; Fumarate reduction ; Nitrate reduction ; Aspartate fermentation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An anaerobic continuous culture study was made with Campylobacter spec. to determine growth yields under various growth conditions. The growth media contained 0.1% (w/v) yeast extract as carbon source. When grown in an aspartate-limited culture Y asp max was 4.6. Inclusion of formate in the culture medium hardly affected the true growth yield. The number of ATP equivalents generated in the fumaratereductase system was 0.66 and the Y ATP max was 7.0. In the nitrate reduction with formate 1.7 ATP equivalents were generated, and a YNO 3- max of 12.2 was observed. The true growth yield obtained with a mixture of lactate and aspartate was lower than that found with aspartate alone.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 117 (1978), S. 109-114 
    ISSN: 1432-072X
    Keywords: Aspartate fermentation ; Fumarate reductase ; Campylobacter ; Anaerobic electron transport
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In the fermentation ofl-aspartate by a free-livingCampylobacter spec., the products formed were acetate, succinate, carbon dioxide and ammonia. The oxidative part of the fermentation pathway yielded acetate, succinate, carbon dioxide and ammonia, and the reductive part gave rise to the formation of succinate and ammonia. When grown anaerobically with aspartate, cells contained cytochromesb andc as well as menaquinone. Reduced cytochromeb, but not reduced cytochromec could be reoxidized by fumarate. In the presence of nitrate, 90% of the available electrons were transferred to nitrate, which was reduced to nitrite; the remainder was transported via the fumarate reductase system. Cells grown with aspartate and excess of formate converted aspartate quantitatively to succinate.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 119 (1978), S. 99-102 
    ISSN: 1432-072X
    Keywords: Campylobacter ; Interspecies hydrogen transfer ; Fumarate reduction ; Nitrate reduction ; Thiosulphate reduction ; Sulphur reduction ; Knallgas reaction ; Hydrogen oxidation ; Formate oxidation ; Sulphide production ; Clostridium cochlearium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A free-living aspartate-fermenting Campylobacter spec. was shown to utilize hydrogen produced in mixed culture by Clostridium cochlearium from glutamate. Resting cells of Campylobacter were shown to reduce aspartate, fumarate and malate as well as nitrate, nitrite, hydroxylamine, sulphite, thiosulphate and elemental sulphur with molecular hydrogen. Growth of Campylobacter spec. was demonstrated with formate as electron donor and nitrate, thiosulphate, elemental sulphur or oxygen as electron acceptor in the presence of acetate as carbon source.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 120 (1979), S. 61-66 
    ISSN: 1432-072X
    Keywords: Clostridium cochlearium ; Clostridium tetanomorphum ; Glutamate fermentation ; Anaerobic mixed cultures
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Clostridium cochlearium could be reproducibly enriched in an L-aspartate- and L-glutamate-limited, anaerobic chemostat inoculated with anaerobic sludge. L-glutamate, L-glutamine and L-histidine were the only fermentable substrates. Less specialised clostridia of the C. tetanomorphum type could only be isolated from batch enrichments with L-glutamate and L-aspartate as energy sources. Competition experiments with C. cochlearium and C. tetanomorphum in a L-glutamate-limited chemostat resulted in the selective elimination of the latter species. Addition of glucose to the medium resulted in coexistence of both species. The molar growth yields for L-glutamate at different dilution rates at 30°C were determined for both species. The maximum specific growth rates on L-glutamate were 0.55 h-1 for C. cochlearium and 0.35 h-1 for C. tetanomorphum.
    Type of Medium: Electronic Resource
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