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  • 1975-1979  (2)
Document type
Publisher
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Year
  • 1
    Electronic Resource
    Electronic Resource
    Restriction and modification in Bacillus species (1977)
    Springer
    Molecular genetics and genomics 152 (1977), S. 65-69 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Host specific restriction was detected in 13 Bacillus strains, when 63 strains of Bacillus subtilis and 15 other Bacillus strains were tested with phage Φ105C. These 13 strains were classified into 8 groups (M,H,C,N,E,F,G,P) by the type of restriction. M-type strains (B. subtilis Marburg 168, its derivatives, and two other strains) showed relatively weak restriction, restricting Φ105C from other groups of Bacillus by ratios of 10-1 to 10-3. Strains of groups H,C,N,E,F,G, and P restricted Φ105C from other groups by ratios of 10-2 to 10-8. It was confirmed with some of the strains that type-specific modification was endowed only by the last host. Furthermore, we isolated one restriction deficient mutant of B. subtilis marburg 168-YS11, which had also lost its modification phenotype.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00264941
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Molecular cloning and in vitro transcription of Bacillus subtilis plasmid in Escherichia coli (1977)
    Springer
    Molecular genetics and genomics 157 (1977), S. 175-182 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A composite plasmid (pAT2010) has been constructed in vitro from RSF2124 and Bacillus subtilis IFO3022 plasmid (pAT1060) by covalent joining of the two DNA molecules by means of Escherichia coli DNA ligase through the cohesive ends generated by restriction endonuclease RI (EcoRI) cleavage. The composite plasmid was selected by transformation of E. coli C600 r −m− with the ligated mixture affer enrichment for composite plasmid by preparative agarose gel electrophoresis, and plating of the transformants on a medium containing ampicillin and colicin El. Treatment of the composite plasmid with EcoRI yielded two fragments corresponding to the linear forms of the parental plasmids. The composite plasmids replicated as biologically functionally units in E. coli, and expressed genetic information carried by RSF2124. In the presence of chloramphenicol, the composite plasmids continued to replicate and the copy number gradually increased. Such nature of replication in the presence of chloramphenicol is characteristic to RSF2124 derived from colicin El factor, and so it is suggested that the replicator of RSF2124 is functional in the composite plasmid. The composite plasmid was found to synthesize mRNA of B. subtilis plasmid in cell-free extracts of E. coli, by hybridization of the mRNA to the original plasmid DNA of pAT1060.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00267395
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    Paper (German National Licenses)
    Fulltext
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