ISSN:
1432-072X
Keywords:
Cytochrome c 3
;
Sulfate-reducing bacteria
;
Colloidal sulfur reduction
;
Hydrogen sulfide
;
Desulfovibrio
;
Chloramphenicol
;
Structure-function
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Cytochromes c 3 of different strains of sulfatereducing bacteria have been purified and tested for their capacity to reduce colloidal sulfur to hydrogen sulfide. The results are in good agreement with the activities reported for the whole cells. Cytochrome c 3 is the sulfur reductase of some strains of sulfate-reducing bacteria such as Desulfovibrio desulfuricans Norway 4 and sulfate-reducing bacterium strain 9974 from which the sulfur reductase activity can be purified with the cytochrome c 3. In contrast, Desulfovibrio vulgaris Hildenborough cytochrome c 3 is inhibited by the product of the reaction namely hydrogen sulfide. Chloramphenicol has no effect on the sulfur reductase activity of D. desulfuricans Norway 4 when resting cells grown on lactate-sulfate medium are put in the presence of colloidal sulfur. This shows that the sulfur reductase activity is constitutive and corresponds to the fact that colloidal sulfur grown cells do not contain more cytochrome c 3 (or another sulfur reductase) than lactate-sulfate-grown cells.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00425065
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