In:
European Journal of Immunology, Wiley, Vol. 9, No. 10 ( 1979-10), p. 815-820
Abstract:
Chimeric mice were constructed by injection of thymus cells into nu/nu mice. The thymus cells carried a different immunoglobulin (Ig) heavy chain linkage group than the cells of the recipients. The chimeric animals were then immunized with the hapten (4‐hydroxy‐3‐nitro‐phenyl)acetyl (NP) on an appropriate carrier, and NP‐binding receptor molecules were isolated from the spleen cells of the animals. Receptor molecules and antibodies were analyzed for the presence of two Ig heavy chain variable region (V H ) markers which are specific for the anti‐NP response of mice carrying the Ig b allogroup, namely the NP b idiotpye and the heteroclitic fine specificity of hapten binding. Two types of receptor molecules are obtained by our method, those carrying determinants of Ig constant domains (anti‐Ig + receptors) and others lacking such determinants (anti‐Ig − receptors). The two V H region markers were found on anti‐Ig − receptors but not on anti‐Ig + receptors and humoral antibodies when the T cells in the chimeric mice carried the Ig b allogroup, whereas the B cells were Ig a/a . Conversely, anti‐Ig + receptors and humoral antibodies but not anti‐Ig − receptors, expressed the two markers when the B cells of the chimeras carried the Ig b and the T cells the Ig a allogroup. From these results and previous data showing that anti‐Ig − receptors are enriched together with T lymphocytes and anti‐Ig + receptors together with B cells, we conclude that the V H ‐bearing anti‐Ig − receptors are not only present on T cells, but are indeed synthesized by these cells.
Type of Medium:
Online Resource
ISSN:
0014-2980
,
1521-4141
DOI:
10.1002/eji.1830091013
Language:
English
Publisher:
Wiley
Publication Date:
1979
detail.hit.zdb_id:
1491907-2
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