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  • Wiley-Blackwell  (2)
  • 1975-1979  (2)
  • 1
    Electronic Resource
    Electronic Resource
    An elastic model of the large-scale structure of duplex DNA (1979)
    New York : Wiley-Blackwell
    Biopolymers 18 (1979), S. 609-623 
    Details
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A general model for the large-scale, time-independent structure of duplex DNA is developed based on elastic considerations. The general conditions of elastic equilibrium are given. These equations are solved for the equilibrium shape of stressed duplex DNA, based on the assumption that the double helix behaves mechanically as a symmetric, linearly elastic rod. It is shown that, in general, two orders of superhelicity will arise at equilibrium. Several possible applications of this approach to the supercoiling of closed circular DNA are described.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bip.1979.360180310
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Scanning electron microscopy of cardiac endothelium of the dogSupported by a grant from the United States Public Health Service, NS 09363 from the Institute of Neurological Diseases and Stroke. (1975)
    New York, NY [u.a.] : Wiley-Blackwell
    American Journal of Anatomy 142 (1975), S. 137-157 
    Details
    ISSN: 0002-9106
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Tissue obtained from young dogs was fixed in buffered aldehydes by vascular perfusion or direct immersion. Selected hearts were maintained in modified mammalian Ringer's solution for three to five minutes prior to fixation. The chambers of the heart and related valves were exposed by dissection and prepared by routine techniques for observation by scanning electron microscopy (SEM). Subsequent to SEM studies, selected specimens were embedded in Epon 812 and sectioned for transmission electron microscopy (TEM).The cardiac endothelium, when fixed immediately in buffered aldehydes, presents an essentially invariable surface throughout the interior of the heart. The predominant nuclear bulges and attenuated peripheral plasmalemma are consistently smooth, with occasional marginal ruffles, scattered microvilli and small blebs. Apart from the higher population of nuclear bulges on valvular surfaces, local variations in SEM of endocardium occur in response to the various stages of systole and diastole encountered. These physiological changes do not produce microappendages.Immersion for three to five minutes in (Chenoweth's) Ringer's solution, prior to fixation, produces a substantial population of microappendages. The cellular surface acquires a swirled appearance erupting in microvilli, blebs and ruffles. These exhibit considerable pleomorphism.There is great lability of the endocardial surface in response to a classic “holding solution” widely used in preparatory techniques. In preparing soft tissues for SEM caution must be used if physiological “holding solutions” are used prior to fixation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aja.1001420202
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    Paper (German National Licenses)
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