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  • American Society for Microbiology  (30)
  • 1975-1979  (30)
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  • American Society for Microbiology  (30)
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  • 1975-1979  (30)
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Subjects(RVK)
  • 1
    Online Resource
    Online Resource
    American Society for Microbiology ; 1975
    In:  Journal of Bacteriology Vol. 124, No. 3 ( 1975-12), p. 1288-1294
    In: Journal of Bacteriology, American Society for Microbiology, Vol. 124, No. 3 ( 1975-12), p. 1288-1294
    Abstract: It is known that in Escherichia coli two dehydrogenases of the flavoprotein kind can participate in the transfer of hydrogens from sn-glycerol 3-phosphate (G3P) to nitrate and that possession of either enzyme is sufficient to permit anaerobic growth on glycerol as carbon source and nitrate as hydrogen acceptor. Results from this study show that under such a growth condition a protein with light-absorption characteristics of cytochrome b1 is induced. If G3P, nitrate, and adenosine diphosphate are added anaerobically to a particulate fraction prepared from these cells, four reactions can be detected: (i) the reduction of the cytochrome b1-like protein, (ii) the formation of dihydroxyacetone phosphate (DHAP), (iii) the formation of nitrite, and (iv) the generation of adenosine 5'-triphosphate (ATP). The anaerobic G3P dehydrogenase system can yield an ATP-DHAP (or ATP-nitrite) molar ratio of about 0.2, whereas the aerobic G3P dehydrogenase system can yield a corresponding ratio of about 0.3. The hydrogen transfer activity is sensitive to respiratory inhibitors such as cyanide, Rotenone, and 2-heptyl-4-hydroxyquinoline-N-oxide.
    Type of Medium: Online Resource
    ISSN: 0021-9193 , 1098-5530
    Language: English
    Publisher: American Society for Microbiology
    Publication Date: 1975
    detail.hit.zdb_id: 1481988-0
    SSG: 12
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  • 2
    Online Resource
    Online Resource
    American Society for Microbiology ; 1975
    In:  Journal of Bacteriology Vol. 124, No. 3 ( 1975-12), p. 1282-1287
    In: Journal of Bacteriology, American Society for Microbiology, Vol. 124, No. 3 ( 1975-12), p. 1282-1287
    Abstract: A particulate subcellular fraction from Escherichia coli K-12 induced in anaerobic sn-glycerol 3-phosphate (G3P) dehydrogenase and fumarate reductase can catalyze under anaerobic conditions the transfer of hydrogens from G3P to fumarate, with attendant generation of high-energy phosphate. The phsophorylation process is more sensitive than the transhydrogenation process to inhibition by the detergent Triton X-100. The same is true with respect to sensitivity to sodium azide, carbonyl cyanide m-chlorophenylhydrazone and N,N'-dicyclohexylcarbodiimide. Such a preparation derived from cells with beta-galactoside permease can accumulate thiomethyl beta-D-galactoside anaerobically, and the accumulation can be stimulated twofold by adding G3P and fumarate. Mutants lacking the membrane-associated Mg2+-dependent adenosine triphosphatase cannot grow anaerobically on glycerol with fumarate as the hydrogen acceptor, although they can grow aerobically on glycerol alone.
    Type of Medium: Online Resource
    ISSN: 0021-9193 , 1098-5530
    Language: English
    Publisher: American Society for Microbiology
    Publication Date: 1975
    detail.hit.zdb_id: 1481988-0
    SSG: 12
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  • 3
    Online Resource
    Online Resource
    American Society for Microbiology ; 1976
    In:  Applied and Environmental Microbiology Vol. 32, No. 2 ( 1976-08), p. 298-299
    In: Applied and Environmental Microbiology, American Society for Microbiology, Vol. 32, No. 2 ( 1976-08), p. 298-299
    Abstract: The crystalline compound produced in large quantity in liquid medium by Aspergillus parasiticus UNBF A12, a high aflatoxin-producing strain isolated from the air in the Federal District of Brazil, was identified as kojic acid. The effect of pH on the production of crystalline kojic acid and aflatoxins by the strain was studied. Fourteen single spore isolates were evaluated for their capacity to produce kojic acid crystals and aflatoxins.
    Type of Medium: Online Resource
    ISSN: 0099-2240 , 1098-5336
    RVK:
    Language: English
    Publisher: American Society for Microbiology
    Publication Date: 1976
    detail.hit.zdb_id: 223011-2
    detail.hit.zdb_id: 1478346-0
    SSG: 12
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  • 4
    Online Resource
    Online Resource
    American Society for Microbiology ; 1979
    In:  Antimicrobial Agents and Chemotherapy Vol. 16, No. 1 ( 1979-07), p. 92-97
    In: Antimicrobial Agents and Chemotherapy, American Society for Microbiology, Vol. 16, No. 1 ( 1979-07), p. 92-97
    Abstract: The antiviral effect of the nucleoside analog 5-iodo-5′-amino-2′,5′-dideoxyuridine (AIU) was tested with three isolates of varicella-zoster virus (VZV). AIU concentrations of 10 to 800 μM (3.5 to 288 μg/ml) reduced the number of plaques produced by VZV-infected cells and cell-free VZV from approximately 30 to 95%. Smaller plaque size was also observed in the presence of AIU. AIU was less effective than arabinofuranosylthymine in reducing VZV-induced plaques since as little as 5 μg of arabinofuranosylthymine per ml completely blocked plaque formation by cell-free VZV. Toxicity assays with human diploid embryo fibroblast cells were also carried out. Drug concentrations as high as 800 μM were not toxic to human diploid embryo fibroblast cells as determined by radiolabeling of cell deoxyribonucleic acid, ribonucleic acid, and protein.
    Type of Medium: Online Resource
    ISSN: 0066-4804 , 1098-6596
    RVK:
    Language: English
    Publisher: American Society for Microbiology
    Publication Date: 1979
    detail.hit.zdb_id: 1496156-8
    SSG: 12
    SSG: 15,3
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  • 5
    In: Antimicrobial Agents and Chemotherapy, American Society for Microbiology, Vol. 15, No. 3 ( 1979-03), p. 361-367
    Abstract: The avermectins are a complex of chemically related agents which exhibit extraordinarily potent anthelmintic activity. They are produced by a novel species of actinomycete, NRRL 8165, which we have named Streptomyces avermitilis . The morphological and cultural characteristics which differentiate the producing organism from other species are described. The avermectins have been identified as a series of macrocyclic lactone derivatives which, in contrast to the macrolide or polyene antibiotics, lack significant antibacterial or antifungal activity. The avermectin complex is fully active against the gastrointestinal nematode Nematospiroides dubius when fed to infected mice for 6 days at 0.0002% of the diet. Fermentation development, including medium modification and strain selection, resulted in increasing the broth yields from 9 to 500 μg/ml.
    Type of Medium: Online Resource
    ISSN: 0066-4804 , 1098-6596
    RVK:
    Language: English
    Publisher: American Society for Microbiology
    Publication Date: 1979
    detail.hit.zdb_id: 1496156-8
    SSG: 12
    SSG: 15,3
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  • 6
    Online Resource
    Online Resource
    American Society for Microbiology ; 1975
    In:  Journal of Bacteriology Vol. 124, No. 1 ( 1975-10), p. 348-352
    In: Journal of Bacteriology, American Society for Microbiology, Vol. 124, No. 1 ( 1975-10), p. 348-352
    Abstract: Klebsiella aerogenes dissimilates glycerol aerobically via an inducible pathway initiated by an adenosine triphosphate-linked kinase that converts the substrate to sn-glycerol 3-phosphate. Phosphorylated glycerol is then dehydrogenated to dihydroxyacetone phosphate by an enzyme characteristic of a flavoprotein. Anaerobically, the organism dissimilates glycerol via an inducible pathway initiated by a nicotinamide adenine dinucleotide-linked dehydrogenase that converts the substrate to dihydroxyacetone. The keto product is then phosphorylated by another adenosine triphosphate-linked kinase. Two kinds of constitutive mutants have been isolated: one affecting the aerobic and the other the anaerobic pathway.
    Type of Medium: Online Resource
    ISSN: 0021-9193 , 1098-5530
    Language: English
    Publisher: American Society for Microbiology
    Publication Date: 1975
    detail.hit.zdb_id: 1481988-0
    SSG: 12
    Location Call Number Limitation Availability
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  • 7
    Online Resource
    Online Resource
    American Society for Microbiology ; 1976
    In:  Infection and Immunity Vol. 13, No. 6 ( 1976-06), p. 1728-1732
    In: Infection and Immunity, American Society for Microbiology, Vol. 13, No. 6 ( 1976-06), p. 1728-1732
    Abstract: The antigenic activity of 10 Visna polypeptides separated by gel filtration in the presence of 6 M guanidine hydrochloride (GuHCl) was examined with rabbit antisera made specific for Visna virus. The results showed that the first (GuHCl 1) and the ninth (GuHCl 9) polypeptide peak reacted with the antisera when examined in immunodiffusion, passive hemagglutination, and complement fixation tests. Whole virus, GuHCl 1, and GuHCl 9, when tested with the antisera, appeared to be immunologically identical in the immunodiffusion test. However, GuHCl 1 reacted weakly with the antisera by all three techniques as compared with GuHCl 9 and whole virus. GuHCl 9, when subjected to polyacrylamide gel electrophoresis containing 0.1% sodium lauryl sulfate, revealed the presence of one polypeptide with a molecular weight of 25,000. By the same method, GuHCl 1 was found to contain an aggregate of four different polypeptides, the major one having a molecular weight of 25,000. The results indicate that the antigenic activity of both GuHCl 1 and GuHCl 9 was associated with a single polypeptide having a molecular weight of 25,000.
    Type of Medium: Online Resource
    ISSN: 0019-9567 , 1098-5522
    RVK:
    Language: English
    Publisher: American Society for Microbiology
    Publication Date: 1976
    detail.hit.zdb_id: 1483247-1
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  • 8
    Online Resource
    Online Resource
    American Society for Microbiology ; 1977
    In:  Antimicrobial Agents and Chemotherapy Vol. 12, No. 6 ( 1977-12), p. 748-750
    In: Antimicrobial Agents and Chemotherapy, American Society for Microbiology, Vol. 12, No. 6 ( 1977-12), p. 748-750
    Abstract: We studied the effect of methadone, alone and in combination with antimicrobial agents, on two strains each of Staphylococcus aureus, Pseudomonas aeruginosa , and Serratia marcescens isolated from blood streams of parenteral drug abusers with bacterial endocarditis. Methadone has its own antibacterial effect, although at supraphysiological concentrations, and is even synergistic with antimicrobial agents against some organisms. Thus, methadone does not interfere with the antibacterial effects of antibiotics in vitro.
    Type of Medium: Online Resource
    ISSN: 0066-4804 , 1098-6596
    RVK:
    Language: English
    Publisher: American Society for Microbiology
    Publication Date: 1977
    detail.hit.zdb_id: 1496156-8
    SSG: 12
    SSG: 15,3
    Location Call Number Limitation Availability
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  • 9
    Online Resource
    Online Resource
    American Society for Microbiology ; 1977
    In:  Antimicrobial Agents and Chemotherapy Vol. 11, No. 1 ( 1977-01), p. 154-160
    In: Antimicrobial Agents and Chemotherapy, American Society for Microbiology, Vol. 11, No. 1 ( 1977-01), p. 154-160
    Abstract: The effect of amphotericin B (AmB) treatment on the mononuclear phagocyte system of mice was investigated. Peritoneal macrophages from mice that received AmB treatment showed a higher phagocytic and antibacterial activity than those from normal untreated mice. When the levels of macrophage precursor cells in bone marrow and spleen were followed in mice after AmB treatment, an eightfold increase in the splenic content of limited stem cells for both macrophages and granulocytes (colony-forming units in culture) and a threefold increase in the number of pluripotent hemopoietic stem cells (colony-forming units in spleen) were observed on day 4. These were also accompanied by a slight increase in the colony-forming units in spleen and in culture in femoral marrows. AmB was capable of inducing a large number of peritoneal colony-forming cells in the peritoneum, and caused a significant rise in the serum level of colony-stimulating factor. No significant change in the level of blood monocytes was noted, although a transient increase in the proportion of neutrophils was observed within 24 h after AmB treatment.
    Type of Medium: Online Resource
    ISSN: 0066-4804 , 1098-6596
    RVK:
    Language: English
    Publisher: American Society for Microbiology
    Publication Date: 1977
    detail.hit.zdb_id: 1496156-8
    SSG: 12
    SSG: 15,3
    Location Call Number Limitation Availability
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  • 10
    Online Resource
    Online Resource
    American Society for Microbiology ; 1977
    In:  Journal of Virology Vol. 21, No. 2 ( 1977-02), p. 560-564
    In: Journal of Virology, American Society for Microbiology, Vol. 21, No. 2 ( 1977-02), p. 560-564
    Abstract: P1, P2, and Mu lysogens of Escherichia coli reproduce more rapidly than nonlysogens during aerobic growth in glucose-limited chemostats. Thus, prophage-containing stains of E. coli are reproductively more fit than the corresponding nonlysogens. If mixed populations are grown by serial dilution under conditions in which growth is not limited, both the lysogen and nonlysogen manifest identical growth rates. The increased fitness of the lysogens in glucose-limited chemostats correlates with a higher metabolic activity of the lysogen as compared with the nonlysogen during glucose exhaustion. We propose that P1, P2, Mu, and lambda prophage all confer an evolutionarily significant reproductive growth advantage to E. coli lysogenic strains.
    Type of Medium: Online Resource
    ISSN: 0022-538X , 1098-5514
    Language: English
    Publisher: American Society for Microbiology
    Publication Date: 1977
    detail.hit.zdb_id: 1495529-5
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