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Digitale Medien

Terminal direct repeats in a retrovirus-like repeated mouse gene family (1981)

Keshet, Eli ; Shaul, Yosef

[s.l.] : Nature Publishing Group

Nature 289 (1981), S. 83-85

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ISSN: 1476-4687

Quelle: Nature Archives 1869 - 2009

Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik

Notizen: [Auszug] Individual copies of these 'retrovirus-like' genes (referred to as VL30 genes) were cloned from a gene library of the inbred mouse strain BALB/c in bacteriophage l (ref. 1). The construction of the gene library from mouse genomic fragments obtained by a partial EcoRI digestion enabled us to select ...

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1038/289083a0

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Digitale Medien

Analysis of mouse embryonic gene library for the frequency of single and multiple copy genes (1980)

Mory, Yves Y. ; Keshet, Eli ; Ram, Daniela ; [weitere]

Springer

Molecular biology reports 6 (1980), S. 203-208

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ISSN: 1573-4978

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie

Notizen: Abstract A gene library was constructed from embryonic mouse DNA by ligating DNA fragments generated by partialEco RI digestion with Charon 4A vector andin vitro packaging. A special consideration was given to randomization of target DNA. The general applicability of a gene library prepared in this manner was assessed through cloning a variety of genes of known reiteration frequency in the mouse genome. The survey included a single copy gene — C region of the immunoglobulin heavy chain, and genes that appear in more than one copy — V region of the immunoglobulin light chain genes and the endogenous retrovirus related genes. In all cases tested the frequency of clone isolation was in good agreement with the expected incidence based on the number of genome equivalents screened and the reiteration frequency of that particular gene. Moreover, we found no preference with regard to the clonability of genes contained in fragments of a wide-size range.