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  • 1
    Electronic Resource
    Electronic Resource
    Cytochemical localization of pectinase activity in laticifers ofNerium oleander L. (1984)
    Springer
    Protoplasma 119 (1984), S. 74-78 
    Details
    ISSN: 1615-6102
    Keywords: Pectinase ; Cytochemistry ; Nonarticulated laticifer ; Nerium oleander
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A method is described for the cytochemical localization of pectinase activity at the ultrastructural level. The procedure involves the use of Benedict's reagent to form an electron-dense copper precipitate when reacted with reducing sugars liberated from exogenously supplied pectin. Using this technique, pectinase activity was examined in the nonarticulated, branched laticifers ofNerium oleander. Electron opaque crystalline deposits indicating the presence of pectolytic enzymes were identified in laticifer central vacuoles. Smaller amounts of reaction product were distributed along the middle lamella between laticifers and adjacent cells. This report represents the first direct evidence for the involvement of pectinase in intrusive growth of nonarticulated laticifers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01287819
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Videomicroscopy in the study of protoplasmic streaming and cell movement (1981)
    Springer
    Protoplasma 109 (1981), S. 209-216 
    Details
    ISSN: 1615-6102
    Keywords: Amoeboid movement ; Particle motion ; Saltation ; Streaming ; Videomicroscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Various types of cell motility have been observed and analyzed with techniques of increasing sensitivity and sophistication. Photokymography, cinemicrography and laser-Doppler spectroscopy have all made important contributions to our knowledge of cytoplasmic streaming and cell movement. Now videomicroscopy is finding applications in recording and analyzing two different kinds of images. Video intensification microscopy by image intensifiers and silicon intensified target (SIT) video cameras is used to intensify images too dim to be viewed by eye or photographed. On the other hand, video enhanced microscopy uses a less sensitive chalnicon or other vidicon camera with adjustable amplification and offset to enhance the contrast and improve the resolution of microscopes that employ instrumental compensators. Both of these videotechniques have greatly extended the usefulness of the optical microscope: image intensification to brighten dim images and video enhancement to improve the contrast and resolution so that even “submicroscopic” structures and events can be recorded. These video techniques can both be further extended by a frame memory, with which images can be further enhanced by computer processing. Still to be developed, however, are appropriate methods for automatic tracking of particle motions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01287640
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Coelomocyte motility (1983)
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 3 (1983), S. 113-121 
    Details
    ISSN: 0886-1544
    Keywords: coelomocytes ; filopodia ; whole cell translocation ; video microscopy ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: We have utilized a video-enhanced contrast system coupled to a DIC-equipped microscope to examine the motility of both whole coelomocytes and individual filopodia. When the cells are left in diluted coelomic fluid, they exhibit a fibroblast-like mode of translocation across the substrate. These cells extend lamellipodia at their advancing margin and develop retraction fibers at the trailing edge. Filopodia are actively extended from the lamellipodia of the advancing margin. Cells that are washed free of the coelomic fluid and placed in an isotonic buffer lose their ability to translocate. Filopodia on these stationary cells are seen to undergo a series of waving and bending motions. These motions are rapid and result in a filopodium folding back upon itself only to reextend later. Both forms of motility are discussed in light of the existing structural and biochemical knowledge of this and other cell types.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cm.970030202
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    Paper (German National Licenses)
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