In:
Journal of High Resolution Chromatography, Wiley, Vol. 3, No. 11 ( 1980-11), p. 551-567
Abstract:
The point of our published papers since 1957 is reviewed. The relations between the required value of peak resolution, K 1 (or R), and peak separation, K 3 (eqn 9); K 1 and relative accuracy of a peak height quantitative method, P h (eqn. 10); K 1 and relative accuracy of a peak area method, P a , (eqn. 12) at different concentration ratios, ϕ, are derived. The final result in Table 2 shows a large influence of ϕ on the required value of K 1 . The approximately linear relation between peak width and retention value (eqn. 18) exists not only in GC. but also in HPLC. Plate height values H 1 and H ∞ for a solute with capacity ratio, k′, equal to unity or approaching infinity, respectively, are used to evaluate the column efficiency (eqn. 20). The measuring methods (eqn. 21,22,23) and parameters effecting on H 1 and H ∞ are given for GC packed column (eqn. 24), GC open tubular column (eqn. 25) and HPLC (eqn. 26). In the light of this, columns of high efficiency were developed. Some typical chromatograms for high speed analysis and separation of complex mixtures are given.
Type of Medium:
Online Resource
ISSN:
0935-6304
,
1521-4168
DOI:
10.1002/jhrc.1240031104
Language:
English
Publisher:
Wiley
Publication Date:
1980
detail.hit.zdb_id:
1491308-2
detail.hit.zdb_id:
2047990-6
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