In:
International Archives of Allergy and Immunology, S. Karger AG, Vol. 72, No. 4 ( 1983), p. 373-376
Kurzfassung:
After guinea pigs were exposed to histamine or acetylcholine aerosol for 1 week, alpha 〈 sub 〉 1 〈 /sub 〉 - and beta-adrenergic and cholinergic muscarinic receptors on the lung membranes were measured by direct binding techniques using 〈 sup 〉 3 〈 /sup 〉 H-prazosin, 〈 i 〉 l 〈 /i 〉 -3H-dihydroalprenolol, and 〈 i 〉 l 〈 /i 〉 - 〈 sup 〉 3 〈 /sup 〉 H-quinuclidinyl benzilate, respectively, and adenylate cyclase responses to /-isoproterenol (10 〈 sup 〉 –– 〈 /sup 〉 〈 sup 〉 5 〈 /sup 〉 to 10 〈 sup 〉 ––8 〈 /sup 〉 〈 i 〉 M 〈 /i 〉 ) and NaF (20 m 〈 i 〉 M 〈 /i 〉 ) were also examined on the membranes. After the inhalation, the number of beta-adrenergic receptors was decreased by about 25% without a significant change in binding affinity for 〈 i 〉 l 〈 /i 〉 - 〈 sup 〉 3 〈 /sup 〉 H-dihydroalprenolol, while the number and the affinity of alphar adrenergic and muscarinic receptors on the membranes were not significantly different from those in the control animals. When compared with the control animals, the lung membranes showed a reduced adenylate cyclase response to 〈 i 〉 l 〈 /i 〉 -isoproterenol, and the response to 10 〈 sup 〉 ––5 〈 /sup 〉 〈 i 〉 M 〈 /i 〉 of 〈 i 〉 l 〈 /i 〉 -isoproterenol was significantly decreased by 11 % in the lung membranes. The histamine inhalation showed no significant effect on basal adenylate cyclase activity and adenylate cyclase response to NaF.
Materialart:
Online-Ressource
ISSN:
1018-2438
,
1423-0097
Sprache:
Englisch
Verlag:
S. Karger AG
Publikationsdatum:
1983
ZDB Id:
1482722-0
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