In:
Acta Endocrinologica, Oxford University Press (OUP), Vol. 98, No. 3 ( 1981-11), p. 352-357
Abstract:
Serum activity that stimulates DNA synthesis was determined in 130 patients of short stature, 6 with acromegaly, and 5 with anorexia nervosa, using a technique to measure [ 3 H]thymidine incorporation into cultured human fibroblasts. The stimulation of thymidine incorporation into the fibroblasts, abbreviated as thymidine incorporation activity (TIA), was significantly lower than normal ( P 〈 0.001) in 18 untreated children with GH-deficiency (0.63 ± 0.14 U/ml, mean ± sd ), and in 5 patients with anorexia nervosa (0.58 ± 0.18), but higher than normal ( P 〈 0.05) in 5 untreated acromegalic patients (1.30 ± 0.15). Pooled control serum from normal adult subjects was defined as having activity of 1 unit/ml of TIA. The mean TIA level of 6 untreated children with GH deficiency increased to normal (0.99 ± 0.26) in the last month of treatment with human growth hormone (KABI-hGH), and that of 5 acromegalic patients decreased to normal or less (0.89 ± 0.20) after transsphenoidal hypophysectomy. There was a good correlation (r = 0.54, P 〈 0.001) between the TIA level and increase in height of 47 GH-deficient children treated with hGH. The TIA values in 22 blod samples obtained from 6 untreated children with GH-deficiency and 16 GH-deficient children treated with hGH were well correlated with values of measurements of [ 3 H]thymidine incorporation into cultured growing chondrocytes of rabbits (r = 0.65, P 〈 0.001). These results suggest that serum activity to stimulate [ 3 H]thymidine incorporation into human fibroblasts has GH dependency, and should be a reliable index in bioassay of total somatomedin-like bioactivity in serum.
Type of Medium:
Online Resource
ISSN:
0804-4643
,
1479-683X
DOI:
10.1530/acta.0.0980352
Language:
Unknown
Publisher:
Oxford University Press (OUP)
Publication Date:
1981
detail.hit.zdb_id:
1183856-5
detail.hit.zdb_id:
1485160-X
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