In:
International Archives of Allergy and Immunology, S. Karger AG, Vol. 75, No. 2 ( 1984), p. 102-106
Abstract:
We present evidence that murine spleen cells produce a T-cell growth-stimulating factor following oxidation by periodic acid (H 〈 sub 〉 5 〈 /sub 〉 IO 〈 sub 〉 6 〈 /sub 〉 ). The identification of this factor as interleukin 2 (IL-2) is indicated by its ability to support the growth of the IL-2-dependent CT6 cell line. In addition, preliminary analysis shows that H 〈 sub 〉 5 〈 /sub 〉 IO 〈 sub 〉 6 〈 /sub 〉 -stimulated growth factor has biochemical properties similar to IL-2. The time course of H 〈 sub 〉 5 〈 /sub 〉 IO 〈 sub 〉 6 〈 /sub 〉 -induced IL-2-like production by spleen cells was determined. No growth-stimulating activity was detected after 1 h of culture. The peak of periodic acid-induced IL-2-like production was between 18 and 24 h, while the maximum 〈 sup 〉 3 〈 /sup 〉 H-thymidine incorporation in spleen cells occurred at 72 h. Flow cytometry of CT6 cells was used for cell cycle analysis and to demonstrate their stimulation by IL-2-containing supernatants. These results were in agreement with the 〈 sup 〉 3 〈 /sup 〉 H-thymidine incorporation data. Electron microscopy of CT6 cells stimulated by supernatants from concanavalin A- or H 〈 sub 〉 5 〈 /sub 〉 lO 〈 sub 〉 6 〈 /sub 〉 -treated spleen cells showed no differences in their morphology. Degradation of spleen cell sialic acid prior to periodic acid oxidation inhibited IL-2-like production by 84% and inhibited 〈 sup 〉 3 〈 /sup 〉 H-thymidine uptake by 80%.
Type of Medium:
Online Resource
ISSN:
1018-2438
,
1423-0097
Language:
English
Publisher:
S. Karger AG
Publication Date:
1984
detail.hit.zdb_id:
1482722-0
Permalink