In:
Clinical Chemistry, Oxford University Press (OUP), Vol. 28, No. 9 ( 1982-09-01), p. 1844-1847
Abstract:
Release tags are novel compounds made up of three molecular groups: "signal," "release," and "reactivity." The signal group is the detected part of the tag, the release group provides a site for specific covalent cleavage, and the reactivity group attaches the tag to a substance of interest. The general purpose of release tags is to function as molecular labels, analogous to radioisotopes. Release tags also extend the applicability of gas-phase electrophores as signal groups to the analysis of nonvolatile substances. These electrophores are measured with high sensitivity and specificity by gas chromatography with electron-capture detection (GC-ECD). Because extensive multiplicity is potentially available with GC-ECD electrophores, many analogous but distinctive release tags can be used simultaneously as labels in a given assay. In this paper, we use N-pentafluorobenzoylmethionylglycine-N-hydroxysuccinimide ester, our first GC-ECD release tag, to determine thyroxin in serum by a method called "labeling analysis," which involves the principle of isotope-derivative analysis.
Type of Medium:
Online Resource
ISSN:
0009-9147
,
1530-8561
DOI:
10.1093/clinchem/28.9.1844
Language:
English
Publisher:
Oxford University Press (OUP)
Publication Date:
1982
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