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  • Springer  (3)
  • 1980-1984  (3)
Document type
Publisher
Years
Year
  • 1
    Electronic Resource
    Electronic Resource
    Isolation and characterization of spontaneous srl-recA deletion mutants in Escherichia coli K-12 (1984)
    Springer
    Molecular genetics and genomics 196 (1984), S. 356-359 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A method was developed for the isolation of spontaneous mutants of Escherichia coli K-12 with deletions extending from the srl operon to the adjacent recA gene. The srl-recA deletion mutants were extremely sensitive to DNA-damaging agents; unable to support growth of the feckless red gam mutant λbio11; and recombination-deficient in transduction and in conjugation. They therefore resembled recA point mutants such as recA13. The existence of these recA deletion mutants shows that the recA gene is not essential for viability.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00328071
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    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Behaviour of the transposons Tn5 and Tn7 in Xanthomonas campestris pv. campestris (1984)
    Springer
    Molecular genetics and genomics 195 (1984), S. 101-107 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Xanthomonas campestris pv. campestris was tested for its ability to maintain various plasmids after they had been transferred by conjugation from Escherichia coli donors. Broad host-range plasmids belonging to incompatibility groups P and Q could be maintained but X. campestris was unable to support replication of narrow host-range ColE1, pACYC184 and pBR325 replicons. Delivery systems based on E. coli donors of suicide plasmids and on X. campestris Hfrs were used to introduce Tn7 and Tn5 into X. campestris. Tn7 insertions were recovered at high frequency while Tn5 transposed at low frequency. Three auxotrophic Tn5 insertions were isolated but transposition of Tn7 into the X. campestris genome did not generate any auxotrophs. DNA hybridization analysis showed that Tn7 had inserted into the same “hot spot(s)” in all cases tested.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00332731
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Molecular cloning of genes involved in purine biosynthetic and salvage pathways of Salmonella typhimurium (1984)
    Springer
    Molecular genetics and genomics 196 (1984), S. 152-157 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Genes have been cloned from Salmonella typhimurium which when present on the multicopy plasmid pBR322 in the E. coli strain NT31 confer a Gua+ phenotype on this strain. NT31 is a purE gpt double mutant and it was expected that a Gua+ phenotype could be conferred on it by the cloning of either gpt or purE. It was, however found that in addition to these two loci the molecular cloning of another gene, which has been identified as hpt, in pBR322 confers a Gua+ phenotype on NT31. This result is explained by the overproduction of the hpt gene product, hypoxanthine phosphoribosyl transferase, which compensates for the lack of the gpt product guanine-xanthine phosphoribosyl transferase. Restriction analysis of the three loci, gpt, hpt and purE is also presented.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00334108
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    Paper (German National Licenses)
    Fulltext
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