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  • 1
    Electronic Resource
    Electronic Resource
    Nodule-specific expression of a chimaeric soybean leghaemoglobin gene in transgenic Lotus corniculatus (1986)
    [s.l.] : Nature Publishing Group
    Nature 321 (1986), S. 669-674 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] When a chimaeric soybean leghaemoglobin gene was introduced into the genome of another legume species, Lotus corniculatus, nodule-specific expression of the chimaeric gene was found in root nodules formed on fully regenerated plants inoculated with the Lotus microsymbiont, Rhizobium loti. ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/321669a0
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  • 2
    Electronic Resource
    Electronic Resource
    A simple and general plant tissue extraction procedure for two-dimensional gel electrophoresis (1987)
    Springer
    Plant cell reports 6 (1987), S. 77-81 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A fast and simple extraction procedure of plant tissue for two-dimensional gel electrophoresis is presented. The procedure is especially useful for the extraction of plant cell suspension cultures, callus and other plant tissues having a high content of phenol oxidases, polysaccharides, polynucleotides, terpenoids and other substances interfering with isoelectric focusing. Due to the speed of the extraction procedure (about 20 min), large numbers of samples containing only milligram amounts of tissue can be easily processed. The simplicity of the method makes it particularly suitable for the extraction of radiolabeled tissues (35S, 32P). This method is perfectly compatible with silver staining, autoradiography and Western blotting analysis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00269745
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  • 3
    Electronic Resource
    Electronic Resource
    Gene transfer to cereal cells mediated by protoplast transformation (1985)
    Springer
    Molecular genetics and genomics 199 (1985), S. 178-182 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Direct gene transfer to cereal cells was achieved by transformation of protoplasts with naked DNA. Protoplasts isolated from cultured cells of Triticum monococcum were incubated in the presence of polyethylene glycol (PEG) with circular and linear plasmid DNA. The pBR322-derived plasmid, pBL1103-4, contained a selectable chimeric gene comprising the protein coding region of the Tn5 aminogly-coside phosphotransferase type II gene (NPT II), the nopaline synthase promoter (pNOS) and the polyadenylation signal of the octopine synthase gene. Transformed cells were selected in medium containing kanamycin and identified by detection of aminoglycoside phosphotransferase II activity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00330256
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  • 4
    Electronic Resource
    Electronic Resource
    Identification of host range determinants in the Rhizobium species MPIK3030 (1986)
    Springer
    Molecular genetics and genomics 203 (1986), S. 42-48 
    Details
    ISSN: 1617-4623
    Keywords: Host-range ; Tn5 mutagenesis ; hsn ; Nodulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary R. meliloti primarily nodulates Medicago sativa but cannot nodulate Macroptilium atropurpureum. By introducing an 11.4 kb region into R. meliloti from the Symplasmid of Rhizobium strain MPIK3030, the host range of the R. meliloti transconjugants were shown to be extended to M. atropurpureum, one of the hosts of MPIK3030 but not normally nodulated by R. meliloti. The region responsible for host range extension was isolated by mass conjugating a clone bank from MPIK3030 into the R. meliloti wild type, and subsequent screening for nodulation on M. atropurpureum. Using deleted derivatives of a plasmid reisolated from endosymbiotic bacteria, the host range region was further narrowed down to three EcoRI fragments. Tn5 mutagenesis allowed the isolation of three discrete regions on an 11.4 kb section, which are involved in the extension of host range to M. atropurpureum. Finally, complementation experiments performed with R. meliloti common nod and hsn mutants indicated that none of the genes involved in the early steps of nodulation, including host-range functions, can be complemented by genes carried on the 11.4 kb fragment derived from MPIK3030.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00330382
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  • 5
    Electronic Resource
    Electronic Resource
    The promoter of TL-DNA gene 5 controls the tissue-specific expression of chimaeric genes carried by a novel type of Agrobacterium binary vector (1986)
    Springer
    Molecular genetics and genomics 204 (1986), S. 383-396 
    Details
    ISSN: 1617-4623
    Keywords: Agrobacterium binary vector system ; Plant transformation ; Tissue specificity ; T-DNA-encoded genes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A “plant gene vector cassette” to be used in combination with various Escherichia coli gene-cloning vectors was constructed. This cassette contains a replication and mobilization unit which allows it to be maintained and to be transferred back and forth between E. coli and Agrobacterium tumefaciens hosts provided these hosts contain plasmid RK2 replication and mobilization helper functions. The cassette also harbors a transferable DNA unit with plant selectable marker genes and cloning sites which can be combined with different bacterial replicons, thus facilitating the reisolation of transferred DNA from transformed plants in E. coli. The vector cassette contains two different promoters derived from the T-DNA-encoded genes 5 and nopaline synthase (NOS). By comparing the levels of expression of the marker enzymes linked to each of these promoter sequences, it was found that the gene 5 promoter is active in a tissue-specific fashion whereas this is not the case for the NOS promoter. This observation provides the first documented instance of a gene derived from a procaryotic host the expression of which is apparently regulated by plant growth factors.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00331014
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  • 6
    Electronic Resource
    Electronic Resource
    The expression of a chimeric soybean beta-tubulin gene in tobacco (1987)
    Springer
    Molecular genetics and genomics 209 (1987), S. 633-633 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00331178
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  • 7
    Electronic Resource
    Electronic Resource
    Primary structure and promoter analysis of leghemoglobin genes of the stem-nodulated tropical legume Sesbania rostrata: Conserved coding sequences, cis-elements and trans-acting factors (1988)
    Springer
    Molecular genetics and genomics 214 (1988), S. 181-191 
    Details
    ISSN: 1617-4623
    Keywords: Leghemoglobin (lb) genes and sequences ; Sesbania rostrata ; Stem and root nodules ; Cis-elements ; Trans-acting factors
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The primary structure of a leghemoglobin (lb) gene from the stem-nodulated, tropical legume Sesbania ostrata and two lb gene promoter regions was analysed. The S. rostrata lb gene structure and Lb amino acid composition were found to be highly conserved with previously described lb genes and Lb proteins. Distinct DNA elements were identified in the S. rostrata lb promoter regions, which share a high degree of homology with cis-active regulatory elements found in the soybean (Glycine max) lbc3 promoter. One conserved DNA element was found to interact specifically with an apparently universal, trans-acting factor present in nuclear extracts of nodules. These results suggest a conserved mechanism for nodule specific induction of lb genes in leguminous plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00337709
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  • 8
    Electronic Resource
    Electronic Resource
    Point mutations in the 23 S rRNA genes of four lincomycin resistant Nicotiana plumbaginifolia mutants could provide new selectable markers for chloroplast transformation (1988)
    Springer
    Molecular genetics and genomics 214 (1988), S. 295-299 
    Details
    ISSN: 1617-4623
    Keywords: Direct DNA sequencing ; 70 S ribosomes ; Antibiotic resistance ; Erythromycin ; Peptidyl transferase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Experiments designed to establish stable chloroplast transformation require selectable marker genes encoded by the chloroplast genome. The antibiotic lincomycin is a specific inhibitor of chloroplast ribosomal activity and is known to bind to the large ribosomal subunit. We have investigated a defined region of the chloroplast 23 S rRNA genes from four lincomycin resistant Nicotiana plumbaginifolia mutants and from wild-type N. plumbaginifolia. The mutants LR415, LR421 and LR446 have A to G transitions at positions equivalent to the nucleotides 2058 and 2059 in the Escherichia coli 23 S rRNA. The mutant, LR400, possesses a G to A transition at a position corresponding to nucleotide 2032 of the E. coli 23 S rRNA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00337724
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  • 9
    Electronic Resource
    Electronic Resource
    Identification and cloning of nodulation genes from the wide host range Rhizobium strain MPIK3030 (1985)
    Springer
    Molecular genetics and genomics 199 (1985), S. 271-278 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A 70 kbp segment of the megaplasmid from a broad host range Rhizobium strain (MPIK3030) was mapped with the aid of cosmid clones made in the vector pJB8. A 7.9 kbp EcoRI fragment from this region, 55 kbp away from the nif gene cluster, was shown to hybridize to the “common” nod genes from R. meliloti. Using several R. meliloti nod probes it was possible to delimit an 830 bp region as being the center of greatest homology. Sequence data from two sections of this region gave a nucleotide homology of 73.7% to the nodC gene of R. meliloti. Using Tn5 mutagenesis a clone was isolated carrying Tn5 in the highly homologous region. When tested on Macroptilium atropurpureum, this MPIK3030 derivative was shown to have a Nod− phenotype. When the wild-type allele was reintroduced into the Tn5 mutant, nodulation was restored. Interspecies complementation also showed that both R. meliloti and Rhizobium sp. MPIK3030 nod regions were able to restore nodulation to Tn5-induced nodC mutants from either strain.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00330269
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  • 10
    Electronic Resource
    Electronic Resource
    Isolation and characterization of a gene from Solanum tuberosum encoding patatin, the major storage protein of potato tubers (1986)
    Springer
    Molecular genetics and genomics 203 (1986), S. 214-220 
    Details
    ISSN: 1617-4623
    Keywords: Patatin ; Gene structure ; S1-Analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The isolation and analysis is described of several cDNA- as well as one genomic clone encoding patatin, the major storage protein from tubers of Solanum tuberosum. The structure of the patatin gene was determined by sequencing a near full-length cDNA clone as well as a genomic fragment of 5.3 kb containing the entire RNA coding part plus about 0.8 kb 5′ upstream sequences. The transcription start site was determined by S1-mapping experiments. An open reading frame of 1,158 nucleotides is found in both the cDNA and the genomic clone. Six introns interrupt the ORF in the genomic sequence. This open reading frame allows for a protein of 42 kd which is 2.5 kd larger than the size of the mature protein. The patatin gene has all the characteristic sequences normally found in eukaryotic genes. A TATA box is found 25 nucleotides upstream of the transcription initiation site and the sequence CAAACT at position -60. In the 3′ region the consensus sequence AATAAA is found 13–18 nucleotides in front of the poly-A-addition site.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00333957
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