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  • 1985-1989  (3)
Document type
Publisher
Years
Year
  • 1
    Electronic Resource
    Electronic Resource
    Transfer of IncW plasmids into methylotrophic bacteria by conjugation and mobilization (1987)
    Springer
    Applied microbiology and biotechnology 26 (1987), S. 147-148 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Escherichia coli IncW plasmid pSa was transferred by conjugation into three Gram-negative strains of methylotrophic bacteria and its physical autonomy, stable maintenance, replication and expression of the majority of its genes in these strains were established. A non-conjugative mini-derivative of pSa, plasmid pGV1106, was introduced into one of the methylotrophs by mobilization.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00253899
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    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Glutamicin CBII, a bacteriocin-like substance produced by (1986)
    Springer
    Antonie van Leeuwenhoek 52 (1986), S. 129-140 
    Details
    ISSN: 1572-9699
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Corynebacterium glutamicum CBII, in the stationary phase of growth, was found to produce spontaneously a substance resembling bacteriocins by its bactericidal properties. This substance designated glutamicin CBII was observed to exhibit bactericidal activity against coryneform bacteria (12 species tested) but not against unrelated gram-positive (3) and gram-negative (3) bacteria, while its action on bacteria with no quite known relatedness to the coryneform group (14) was found to be variable. Glutamicin CBII was partially purified by precipitation with ammonium sulphate (70% saturation), selective heat precipitation and gel chromatography on Sepadex G-50. The antibacterial substance diffused through cellophane membrane with an approximate cut-off of 10000 dalton and its sedimentation coefficient was determined to be 1.1. S by ultracentrifugation. Heating at 100°C for 30 min had no effect on its activity. Glutamicin CBII was proved to be resistant to chloroform, trypsin, chymotrypsin, pronase, and subtilisin. According to its staining behaviour and 1H NMR spectra it probably represents a glycoprotein containing only a minor protein component.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00429316
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Expression of the threonine operon fromEscherichia coli inBrevibacterium flavum andCorynebacterium glutamicum (1989)
    Springer
    Biotechnology letters 11 (1989), S. 231-236 
    Details
    ISSN: 1573-6776
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The threonine operon fromEscherichia coli was cloned in plasmid pBR322, subcloned into the shuttle vector pCEM300 and the resulting recombinant plasmid was transferred intoBrevibacterium flavum andCorynebacterium glutamicum. The expression ofE. coli threonine genes in these coryneform bacteria was demonstrated by complementing thethrA andthrB mutations and by assaying homoserine dehydrogenase activity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01031569
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    Paper (German National Licenses)
    Fulltext
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